Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Neurosci Lett. 2022 Jan 1;766:136352. doi: 10.1016/j.neulet.2021.136352. Epub 2021 Nov 14.
The aim of the current study was to determine effects of the prenatal exposure to crocin in the expression of withdrawal syndrome on reflexive motor behaviors in mice offspring's. Fourteen male mice and 56 adult female mice were randomly divided into seven groups as: control group (morphine-abstinent male and female); group 2, drug-naïve female and morphine-abstinent male; group 3, drug-naïve male and morphine-abstinent females; group 4, drug-naïve male and female. Groups 5-7, were similar to groups 2-4, except crocin (5 mg/kg) were injected to drug-naïve subjects. Following delivery, 20 pups from each litter were selected and behavior and reflexive motor behaviors were determined. Also, blood samples were taken to determine serum antioxidant activity. According to the results, immobility time significantly increased in offspring of the paternal + maternal exposed to morphine swimming test and tail suspension tests (P < 0.05) and significantly decreased in offspring of paternal + maternal exposed to morphine + crocin group (P < 0.05). Ambulation, surface righting, hind-limb suspension, grip strength and front limb suspension significantly decreased in offspring of the mice exposed to morphine (P < 0.05) and significantly improved in offspring of paternal + maternal exposed to morphine + crocin group (P < 0.05). Hind-limb foot angle and negative geotaxis significantly increased in mice with morphine-exposed offspring's (P < 0.05) while improved in offspring of paternal + maternal exposed to morphine + crocin group (P < 0.05). Prenatal exposure to morphine increased Malondialdehyde while decreased Superoxide dismutase, Glutathione peroxidase and total antioxidant status in mice offspring's (P < 0.05) and these results reversed by prenatal exposure to crocin (P < 0.05). In all studied factors, paternal + maternal exposed to morphine + crocin group had better results compared to the other crocin-received drug-naïve groups (P < 0.05). These results suggested prenatal exposure to crocin decreased morphine-induced adverse effect which paternal and maternal exposed to morphine + crocin had the highest prevention against these effects in mice offspring's.
本研究旨在确定孕期摄入西红花酸对小鼠后代戒断综合征反射运动行为表达的影响。将 14 只雄性小鼠和 56 只成年雌性小鼠随机分为 7 组：对照组（吗啡戒断的雄性和雌性）；第 2 组，药物未接触的雌性和吗啡戒断的雄性；第 3 组，药物未接触的雄性和吗啡戒断的雌性；第 4 组，药物未接触的雄性和雌性。第 5-7 组与第 2-4 组相似，只是将西红花酸（5mg/kg）注射到药物未接触的受试对象中。分娩后，从每窝中选择 20 只幼崽，确定行为和反射运动行为。此外，还采集血液样本以测定血清抗氧化活性。结果表明，在吗啡游泳试验和悬尾试验中，雄性+雌性暴露于吗啡的后代的不动时间显著增加（P<0.05），而雄性+雌性暴露于吗啡+西红花酸组的后代的不动时间显著减少（P<0.05）。在暴露于吗啡的小鼠的后代中，活动、表面翻身、后肢悬挂、握力和前肢悬挂显著降低（P<0.05），而在雄性+雌性暴露于吗啡+西红花酸组的后代中显著改善（P<0.05）。在暴露于吗啡的后代中，后肢足角和负趋地性显著增加（P<0.05），而在雄性+雌性暴露于吗啡+西红花酸组的后代中则有所改善（P<0.05）。在暴露于吗啡的小鼠的后代中，丙二醛增加，而超氧化物歧化酶、谷胱甘肽过氧化物酶和总抗氧化状态降低（P<0.05），而这些结果通过产前暴露于西红花酸得到逆转（P<0.05）。在所有研究的因素中，雄性+雌性暴露于吗啡+西红花酸组的结果优于其他接受西红花酸的药物未接触组（P<0.05）。这些结果表明，产前暴露于西红花酸可降低吗啡引起的不良反应，而雄性和雌性暴露于吗啡+西红花酸的后代对这些影响的预防效果最高。
