Graduate student, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Division of Physiology, Department of Basic Sciences, Faculty of Veterinary Medicine, Science and Research Branch, Islamic Azad University, Tehran, Iran.
Int J Dev Neurosci. 2022 Aug;82(5):397-406. doi: 10.1002/jdn.10187. Epub 2022 Apr 29.
Tadalafil has positive effects on neurodevelopment and antioxidant defense system, but there is no information for its possible role during gestation on reflexive motor behavior in offspring. So current study determined the effect of prenatal exposure to the Tadalafil on reflexive motor behaviors and antioxidant activity in mice offspring and antidepressive behaviors in postpartum dams. Forty pregnant female NMRI mice were allocated into four groups. In control group, mice received water while in Groups 2-4, female mice orally gavage with Tadalafil (0.4, 0.8, and 1.6 mg/kg) at gestation day (GD) 5, 8, 11, 14, and 17, respectively. Following delivery, pups were selected and reflexive motor behaviors determined using ambulation, hind-limb foot angle, surface righting, hind-limb strength, grip strength, front-limb suspension, and negative geotaxis tests. Also, serum malondialdehyde (MDA), superoxide dismutase (SOD), glutathione peroxidase (GPx), and total antioxidant status (TAS) were determined in offspring. On Day 2 postpartum, antidepressant activity of Tadalafil was determined by open field test (OFT), rotarod, forced swimming test (FST), and tail suspension test (TST) in dams. Based on the findings, maternal exposure to Tadalafil improved ambulation score, hind-limb suspension score, grip strength, and front-limb suspension in offspring (P < 0.05). Prenatal exposure to Tadalafil decreased surface righting, hind-limb foot angle, and negative geotaxis in offspring (P < 0.05). Tadalafil decreased blood MDA and increased SOD and GPx levels in offspring (P < 0.05). Tadalafil significantly decreased immobility time in FST and TST and increased number of squares crossed in OFT and spending time on rotarod on postpartum mice (P < 0.05). These results suggested that parental exposure of Tadalafil has positive effect on reflexive motor and postpartum behaviors.
他达拉非对神经发育和抗氧化防御系统有积极影响，但没有关于其在妊娠期对后代反射运动行为可能作用的信息。因此，目前的研究旨在确定产前暴露于他达拉非对小鼠后代反射运动行为和抗氧化活性以及产后母鼠抗抑郁行为的影响。将 40 只怀孕的 NMRI 雌性小鼠分为四组。在对照组中，小鼠给予水，而在第 2-4 组中，雌性小鼠在妊娠第 5、8、11、14 和 17 天分别经口灌胃给予他达拉非（0.4、0.8 和 1.6mg/kg）。分娩后，选择幼仔并通过步行、后肢足角度、表面翻正、后肢力量、握力、前肢悬挂和负趋地性试验测定反射运动行为。还测定了幼仔血清丙二醛（MDA）、超氧化物歧化酶（SOD）、谷胱甘肽过氧化物酶（GPx）和总抗氧化状态（TAS）。产后第 2 天，通过旷场试验（OFT）、转棒试验、强迫游泳试验（FST）和悬尾试验（TST）测定他达拉非对母鼠的抗抑郁活性。基于这些发现，母体暴露于他达拉非可改善幼仔的步行评分、后肢悬挂评分、握力和前肢悬挂评分（P<0.05）。产前暴露于他达拉非可降低幼仔的表面翻正、后肢足角度和负趋地性（P<0.05）。他达拉非可降低幼仔血液中的 MDA 并增加 SOD 和 GPx 水平（P<0.05）。他达拉非可显著减少 FST 和 TST 中的不动时间，增加 OFT 中的穿越方格数和旋转棒上的停留时间产后的老鼠（P<0.05）。这些结果表明，父母暴露于他达拉非对反射运动和产后行为有积极影响。
