Special Centre for Nanoscience, Jawaharlal Nehru University, New Delhi 110067, India.
Special Centre for Nanoscience, Jawaharlal Nehru University, New Delhi 110067, India.
Food Chem. 2022 Feb 15;370:131312. doi: 10.1016/j.foodchem.2021.131312. Epub 2021 Oct 5.
A rapid and sensitive hybrid nanosensor was reported with a colorimetric and fluorometric readout system using graphene quantum dots (GQDs) and gold nanoparticles (AuNPs) for the detection of gentamicin (GENTA) in milk and egg samples. In the presence of AuNPs, the fluorescence (FL) intensity of GQDs gradually decreased due to the inner filter effect (IFE) of AuNPs on the FL intensity of GQDs. In addition of GENTA into AuNPs, AuNPs get aggregated and changed their color from red to blue, due to which the IFE of AuNPs reduced and hence, enhanced FL intensity of GQDs with varying concentration of GENTA from 1.03 to 16.55 μM with a lower detection limit of 0.422 μM and 0.493 μM in colorimetric and fluorimetric systems, respectively. The practical application of the developed nanosensor is tested against the real spiked sample. The excellent and straightforward recovery efficiency of the developed nanosensor for the determination of GENTA and can be used as a promising and adaptable tool for speedy monitoring of food quantity.
本文报道了一种快速灵敏的杂交纳米传感器，该传感器具有比色和荧光读出系统，使用石墨烯量子点（GQDs）和金纳米粒子（AuNPs）检测牛奶和鸡蛋样品中的庆大霉素（GENTA）。在 AuNPs 的存在下，由于 AuNPs 对 GQDs 的荧光（FL）强度的内滤效应（IFE），GQDs 的 FL 强度逐渐降低。此外，当 GENTA 加入到 AuNPs 中时，AuNPs 聚集并改变颜色从红色变为蓝色，由于 AuNPs 的 IFE 降低，因此，随着 GENTA 浓度从 1.03 到 16.55 μM 的变化，GQDs 的 FL 强度增强，在比色和荧光系统中的检测限分别为 0.422 μM 和 0.493 μM。该纳米传感器的实际应用已针对实际加标样品进行了测试。开发的纳米传感器对 GENTA 的测定具有优异和直接的回收率，可作为一种有前途和适应性强的工具，用于快速监测食品数量。
