Shenzhen Key Laboratory of Special Functional Materials & Guangdong Research Center for Interfacial Engineering of Functional Materials, College of Materials Science and Engineering, Shenzhen University, Shenzhen 518060, China.
Fujian Provincial Key Laboratory of Quantum Manipulation and New Energy Materials, College of Physics and Energy, Fujian Normal University, Fuzhou 350117, China.
Sensors (Basel). 2024 Jan 24;24(3):773. doi: 10.3390/s24030773.
In this work, we report a new concept of upconversion-powered photoelectrochemical (PEC) bioanalysis. The proof-of-concept involves a PEC bionanosystem comprising a NaYF:Yb,Tm@NaYF upconversion nanoparticles (UCNPs) reporter, which is confined by DNA hybridization on a CdS quantum dots (QDs)/indium tin oxide (ITO) photoelectrode. The CdS QD-modified ITO electrode was powered by upconversion absorption together with energy transfer effect through UCNPs for a stable photocurrent generation. By measuring the photocurrent change, the target DNA could be detected in a specific and sensitive way with a wide linear range from 10 pM to 1 μM and a low detection limit of 0.1 pM. This work exploited the use of UCNPs as signal reporters and realized upconversion-powered PEC bioanalysis. Given the diversity of UCNPs, we believe it will offer a new perspective for the development of advanced upconversion-powered PEC bioanalysis.
在这项工作中，我们报告了一种上转换光电化学（PEC）生物分析的新概念。该概念验证涉及一个 PEC 生物纳米系统，该系统由上转换纳米粒子（UCNPs）报告器组成，该报告器通过 DNA 杂交被限制在 CdS 量子点（QDs）/氧化铟锡（ITO）光电电极上。CdS QD 修饰的 ITO 电极通过上转换吸收以及通过 UCNPs 的能量转移效应来供电，以稳定地产生光电流。通过测量光电流的变化，可以以特定且灵敏的方式检测目标 DNA，其线性范围从 10 pM 到 1 μM，检测限低至 0.1 pM。这项工作利用 UCNPs 作为信号报告器，并实现了上转换供电的 PEC 生物分析。鉴于 UCNPs 的多样性，我们相信它将为先进的上转换供电 PEC 生物分析的发展提供新的视角。
