Associations Between the Purinergic Receptor P2X7 and Leprosy Disease.

Leprosy is an infectious disease still highly prevalent in Brazil, having been detected around 27,863 new cases in 2019. Exposure to may not be sufficient to trigger the disease, which seems to be influenced by host immunogenetics to determine resistance or susceptibility. The purinergic receptor P2X7 plays a crucial role in immunity, inflammation, neurological function, bone homeostasis, and neoplasia and is associated with several infectious and non-infectious diseases. Here, we first compare the expression in RNA-seq experiments from 16 leprosy cases and 16 healthy controls to establish the magnitude of allele-specific expression for single-nucleotide polymorphisms of the gene and to determine the level of gene expression in healthy and diseased skin. In addition, we also evaluated the association of two single-nucleotide polymorphisms (c.1513A>C/rs3751143 and c.1068A>G/rs1718119) with leprosy risk. The expression of was found significantly upregulated at macrophage cells from leprosy patients compared with healthy controls, mainly in macrophages from lepromatous patients. Significant risk for leprosy disease was associated with loss function of rs3751143 homozygous mutant CC [CC vs. AA: = 0.001; odds ratio (OR) = 1.676, 95% CI = 1.251-2.247] but not with heterozygous AC (AC vs. AA: = 0.001; OR = 1.429, 95% CI = 1.260-1.621). Contrary, the polymorphic A allele from the gain function of rs1718119 was associated with protection for the development of leprosy, as observed in the dominant model (AA + AG × GG = 0.0028; OR = 0.03516; CI = 0.1801-0.6864). So, our results suggest that the functional P2X7 purinergic receptor may exert a key role in the death inside macrophages and inflammatory response, which is necessary to control the disease.