Prevalence and antimicrobial susceptibility of extended-spectrum beta lactamases-producing and isolated in selected hospitals of Anyigba, Nigeria.

BACKGROUND

and are commonly implicated in urinary tract infections accounting for majority of the antimicrobial resistance encountered in hospitals.

OBJECTIVES

To determine the prevalence and antimicrobial susceptibility of extended-spectrum beta-lactamases (ESBLs) producing and among patients in Anyigba, Nigeria.

METHODS

This hospital-based cross-sectional study was conducted using urine samples from 200 patients of Grimmard Catholic hospital and Maria Goretti hospital. Urine samples were processed to identify ESBL-producing and using standard microbiological techniques. Isolates were then tested against antimicrobial agents.

RESULTS

A total of 156 bacterial isolates were recovered consisting 128 of and 28 of . Extended spectrum beta-lactamases production was observed in 69% of and 31% of . These pathogens were resistant to 3 or more antibiotics. Of the antimicrobials tested, cefotaxime demonstrated the highest rates of resistance (100%) for both ESBL-producing and . Fifty-four isolates of ESBL-producing showed a high level of resistance to amoxicillin clavulanic acid (83.3%), ciprofloxacin (83.3%), and ceftazidime (79.6%). ESBL-positive isolates were highly resistant to ciprofloxacin (75%), and amoxicillin clavulanic acid (83.3%). Cefoxitin (62.5%) and gentamicin (66.7%) showed substantially higher rates of resistance against these isolates while all 24 strains were resistant to imipenem.

CONCLUSION

This study indicated the prevalence of ESBL-positive Gram-negative pathogens in these study sites and also demonstrated their resistance to a few antibiotics. This highlights the need for new antimicrobials that are potent and improved policy on use of antibiotics.