Head of the Department of Oncology and Neurosurgery, University Clinic; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
Neurosurgeon, University Clinic; Privolzhsky Research Medical University, 10/1 Minin and Pozharsky Square, Nizhny Novgorod, 603005, Russia.
Sovrem Tekhnologii Med. 2021;12(6):36-44. doi: 10.17691/stm2020.12.6.05. Epub 2020 Dec 28.
is to evaluate biocompatibility of a novel hybrid polyoligomer in and models.
Cytotoxicity of the material was investigated using the MTT assay with human dermal fibroblasts as test cultures. To study direct interaction of the hybrid polyoligomer with cells, the fibroblasts were cultured on the polymer samples for 96 h, the cultures were assessed every 24 h using fluorescence microscopy. To study the tissue reaction in the area of contact with the donor bed and the morphological features of the implanted sample restructuring, a case-control study was performed using a rabbit model. Samples of hybrid polyoligomer were implanted into the bone defect formed in the left iliac crest in 10 rabbits. In the control group, the prepared allograft samples were transplanted into similar defects in 10 animals. The rabbits were sacrificed 4 and 8 weeks after the operation. The standard morphological methods with hematoxylin and eosin staining and immunohistochemical Ki-67 proliferation marker evaluation were used to assess the state of tissues in the defect area.
The results demonstrate that the hybrid polyoligomer is not cytotoxic (cytotoxicity score 0-1), cells adhere well to its surface, retain their viability and typical morphology throughout the entire observation period. No negative impact of material implantation on the health state and behavior of animals was detected. Morphological examination showed the absence of inflammatory changes, formation of thin-walled capillary vessels, and considerable proliferative activity of mesenchymal cells in the defect area, even though it was more intense than in the control group.
No inflammation signs were detected by 8 week of the experiment. It was defined that new bone was beginning to form. The results of analysis support the conclusion that the developed hybrid materials are prospective for further research as potential bone substitute.
评价新型杂化聚醇在 和 模型中的生物相容性。
采用 MTT 法检测材料的细胞毒性，以人真皮成纤维细胞为试验培养物。为了研究杂化聚醇与细胞的直接相互作用，将成纤维细胞在聚合物样品上培养 96 小时，每隔 24 小时用荧光显微镜评估培养物。为了研究与供体床接触区域的组织反应和植入样品重构的形态特征，使用兔模型进行了病例对照研究。将杂化聚醇样品植入 10 只兔子左侧髂嵴形成的骨缺损中。在对照组中，将制备的同种异体移植物样本移植到 10 只类似缺陷的动物中。手术后 4 周和 8 周处死兔子。采用苏木精-伊红染色和 Ki-67 增殖标志物免疫组化评价标准形态学方法评估缺损区域组织状态。
结果表明，杂化聚醇无细胞毒性（细胞毒性评分 0-1），细胞在其表面黏附良好，整个观察期内保持活力和典型形态。未发现材料植入对动物健康状态和行为的负面影响。形态学检查显示，在缺损区域无炎症变化，形成薄壁毛细血管，间充质细胞增殖活性较高，但较对照组弱。
实验 8 周时未检测到炎症迹象。确定新骨开始形成。分析结果支持以下结论：所开发的杂化材料具有作为潜在骨替代物的进一步研究前景。
