Yudin Vladimir V, Kulikova Tatyana I, Morozov Alexander G, Egorikhina Marfa N, Rubtsova Yulia P, Charykova Irina N, Linkova Daria D, Zaslavskaya Maya I, Farafontova Ekaterina A, Kovylin Roman S, Aleinik Diana Ya, Chesnokov Sergey A
Privolzhsky Research Medical University of the Ministry of Health of the Russian Federation, 10/1, Ploshchad Minina i Pozharskogo, 603005 Nizhny Novgorod, Russia.
Laboratory of Photopolymerization and Polymer Materials, G. A. Razuvaev Institute of Organometallic Chemistry, Russian Academy of Sciences, 49, Tropinina, 603950 Nizhny Novgorod, Russia.
Polymers (Basel). 2024 Jan 30;16(3):379. doi: 10.3390/polym16030379.
Hybrid porous polymers based on poly-EGDMA and polylactide containing vancomycin, the concentration of which in the polymer varied by two orders of magnitude, were synthesized. The processes of polymer biodegradation and vancomycin release were studied in the following model media: phosphate-buffered saline (PBS), trypsin-Versene solution, and trypsin-PBS solution. The maximum antibiotic release was recorded during the first 3 h of extraction. The duration of antibiotic escape from the polymer samples in trypsin-containing media varied from 3 to 22 days, depending on the antibiotic content of the polymer. Keeping samples of the hybrid polymer in trypsin-containing model media resulted in acidification of the solutions-after 45 days, up to a pH of 1.84 in the trypsin-Versene solution and up to pH 1.65 in the trypsin-PBS solution. Here, the time dependences of the vancomycin release from the polymer into the medium and the decrease in pH of the medium correlated. These data are also consistent with the results of a study of the dynamics of sample weight loss during extraction in the examined model media. However, while the polymer porosity increased from ~53 to ~60% the pore size changed insignificantly, over only 10 μm. The polymer samples were characterized by their antibacterial activity against , and this activity persisted for up to 21 days during biodegradation of the material, regardless of the medium type used in model. Surface-dependent human cells (dermal fibroblasts) adhere well, spread out, and maintain high viability on samples of the functionalized hybrid polymer, thus demonstrating its biocompatibility in vitro.
合成了基于聚乙二醇二甲基丙烯酸酯和含有万古霉素的聚丙交酯的杂化多孔聚合物，其中聚合物中万古霉素的浓度变化了两个数量级。在以下模型介质中研究了聚合物的生物降解过程和万古霉素的释放：磷酸盐缓冲盐水（PBS）、胰蛋白酶 - 维尔烯溶液和胰蛋白酶 - PBS溶液。在提取的前3小时记录到最大抗生素释放量。在含胰蛋白酶的介质中，聚合物样品中抗生素释放的持续时间从3天到22天不等，这取决于聚合物中抗生素的含量。将杂化聚合物样品保存在含胰蛋白酶的模型介质中会导致溶液酸化——45天后，胰蛋白酶 - 维尔烯溶液的pH值降至1.84，胰蛋白酶 - PBS溶液的pH值降至1.65。在此，万古霉素从聚合物释放到介质中的时间依赖性与介质pH值的降低相关。这些数据也与在研究的模型介质中提取过程中样品重量损失动力学的研究结果一致。然而，虽然聚合物的孔隙率从约53%增加到约60%，但孔径变化不明显，仅超过10μm。聚合物样品对……具有抗菌活性，并且在材料生物降解过程中，这种活性持续长达21天，与模型中使用的介质类型无关。表面依赖性人类细胞（真皮成纤维细胞）在功能化杂化聚合物样品上粘附良好、铺展并保持高活力，从而证明了其在体外的生物相容性。
