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从韩国本土小牛分离出的潜在益生菌菌株1394N20的全基因组序列

Complete genome sequence of 1394N20, a potential probiotic strain, isolated from a Hanwoo calf.

作者信息

Oh Young Joon, Kim Joon Yong, Lee Jieun, Lim Seul Ki, Yu Dohyeon, Oh Yeon-Su, Park Jinho, Choi Hak-Jong

机构信息

Research and Development Division, World Institute of Kimchi, Gwangju 61755, Korea.

SME Service Department, Strategy and Planning Division, World Institute of Kimchi, Gwangju 61755, Korea.

出版信息

J Anim Sci Technol. 2021 Sep;63(5):1207-1210. doi: 10.5187/jast.2021.e100. Epub 2021 Sep 30.

Abstract

are known to exist in the intestinal flora of healthy cattle or pigs. The strain 1394N20 was isolated from the feces of the Hanwoo calf (). The genome of strain 1394N20 consists of a single circular chromosome (2,176,326 bp) with overall guanine + cytosine content of 37.8 mol%. Moreover, 2,281 protein-coding sequences, 15 rRNAs, and 65 tRNAs genes were identified in the chromosome based on the results of annotation. The bacterium has a gene encoding endoglucanase, an enzyme that hydrolyzes the 1,4-β-D-glycosidic linkages in cellulose, hemicellulose, lichenin, and cereal β-D-glucans. Genomic sequencing of strain 1394N20 reveals the immense potential of the strain as a probiotic with nutrient digestibility.

摘要

已知存在于健康牛或猪的肠道菌群中。1394N20菌株是从韩牛犊牛粪便中分离出来的()。1394N20菌株的基因组由一条单一的环状染色体(2,176,326 bp)组成,鸟嘌呤+胞嘧啶的总体含量为37.8 mol%。此外,根据注释结果,在染色体中鉴定出2,281个蛋白质编码序列、15个rRNA和65个tRNA基因。该细菌具有编码内切葡聚糖酶的基因,内切葡聚糖酶是一种能水解纤维素、半纤维素、地衣多糖和谷物β-D-葡聚糖中1,4-β-D-糖苷键的酶。1394N20菌株的基因组测序揭示了该菌株作为具有营养消化能力的益生菌的巨大潜力。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/8351/8564313/844e8dbe4da4/jast-63-5-1207-g1.jpg

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