Shen Jiakun, Zhang Jie, Zhao Ying, Lin Zishen, Ji Linbao, Ma Xi
State Key Laboratory of Animal Nutrition, College of Animal Science and Technology, China Agricultural University, Beijing, China.
School of Public Health, North China University of Science and Technology, Hebei, China.
Front Nutr. 2022 Mar 29;9:846991. doi: 10.3389/fnut.2022.846991. eCollection 2022.
The interaction between exogenous microorganisms and the host has received great attention, and finding new probiotics is always the way to improve the health of humans and animals. () is a kind of that can efficiently utilize starch, as a food and feed additive, it has been widely used for mildew prevention and antibacterial, bacteriostasis, and enzyme production. Herein, a strain of was isolated from the feces of Tibetan weaned piglets, named SLZX20-1. Physiological and biochemical experiments confirmed that it had a fast growth rate and could produce a variety of enzymes, including α-galactosidase, β-galactosidase, α-glucosidase, β-glucosidase, and ferulic acid esterase. In addition, SLZX20-1 exerted antibiotic effects on the growth of () SL1344, () DBS100, () CVCC1791, () CVCC1882, () O157, K88, K99, and 987P, which are closely related to acid productivity, such as lactic acid and acetic acid. co-culture, SLZX20-1 has shown the strong adhesion ability to intestinal porcine epithelial cells (IPEC-J2 cells) and activated IPEC-J2 cells with high expression of host defense peptides (HDPs), such as , and . experiment, intragastric administration, SLZX20-1 significantly improved the feed intake of mice, declined the crypt depth of jejunum and ileum, SLZX20-1 changed the composition of intestinal microbes, especially at the level of colonic genus, the dominant genus was changed from to , which indicated the change of intestinal carbohydrate nutrition. In conclusion, SLZX20-1 showed strong probiotic characteristics, which met with the standard of probiotics and is worth further exploring its impacts on host health and its potential as a candidate strain of probiotics.
外源微生物与宿主之间的相互作用受到了极大关注，寻找新的益生菌一直是改善人类和动物健康的途径。（）是一种能够高效利用淀粉的（物质），作为食品和饲料添加剂，它已被广泛用于防霉、抗菌、抑菌和产酶。在此，从藏猪断奶仔猪粪便中分离出一株（菌株），命名为SLZX20 - 1。生理生化实验证实其生长速度快，能产生多种酶，包括α - 半乳糖苷酶、β - 半乳糖苷酶、α - 葡萄糖苷酶、β - 葡萄糖苷酶和阿魏酸酯酶。此外，SLZX20 - 1对与酸生产力密切相关的（如乳酸和乙酸）（菌株）（）SL1344、（）DBS100、（）CVCC1791、（）CVCC1882、（）O157、K88、K99和987P的生长具有抗菌作用。在共培养中，SLZX20 - 1对猪肠道上皮细胞（IPEC - J2细胞）显示出较强的黏附能力，并激活了高表达宿主防御肽（如（）、（）和（））的IPEC - J2细胞。在实验中，通过灌胃给予SLZX20 - 1，显著提高了小鼠的采食量，降低了空肠和回肠的隐窝深度，SLZX20 - 1改变了肠道微生物的组成，尤其是在结肠属水平，优势属从（）变为（），这表明肠道碳水化合物营养发生了变化。总之，SLZX20 - 1表现出很强的益生菌特性，符合益生菌标准，值得进一步探索其对宿主健康的影响及其作为益生菌候选菌株的潜力。
