Wang Jin, Zhang Jiayu, Zhong Yuting, Qin Liqiang, Li Jianxiang
Department of Toxicology, School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu, China.
Department of Nutrition and Food Hygiene, School of Public Health, Medical College of Soochow University, Suzhou, Jiangsu, China.
J Food Sci. 2021 Dec;86(12):5424-5438. doi: 10.1111/1750-3841.15970. Epub 2021 Nov 18.
Selenium (Se) is a vital trace element in human beings and is essential for protection against oxidative stress. This study aimed to investigate the accumulation and antioxidant effects of two organic seleniums, L-selenomethionine (SM) and L-Se-methylselenocysteine (SMC), through in vivo and in vitro experiments. L02 cells were pretreated with 10 nM SM or SMC for 24 h, followed by exposure to 100 nM of H₂O₂. Cell viability, apoptosis, and antioxidant capacity were detected to evaluate SM and SMC's protective effect. Organic selenium (SM and SMC) and inorganic selenium (sodium selenite, SS) were compared in terms of their in vivo accumulation and antioxidant capacity when supplemented daily and subsequently deprived in SD rats. Our results show that SM or SMC pre-treatment could significantly prevent elevated apoptosis and declined antioxidant ability. We found that organic Se supplementation resulted in higher Se accumulation than inorganic Se in the liver and kidney. The antioxidant capacity of liver and kidney tissues from rats fed with either organic selenium was significantly improved and was higher than that of SS. In summary, this study suggests that organic selenium supplements are more effective in facilitating Se accumulation in liver and kidney, enhancing antioxidant capacities, thereby protecting cells from oxidative stress. PRACTICAL APPLICATION: This study compared the antioxidant capacity of sodium selenite, L-selenomethionine, and L-Se-methylselenocysteine in vitro and in vivo. The results showed that organic selenium has a stronger antioxidant capacity and that significant differences exist in its absorption and conversion in male and female rats. Our results provide theoretical guidance for dietary supplementation of selenium.
硒（Se）是人体必需的微量元素，对抵御氧化应激至关重要。本研究旨在通过体内和体外实验，探究两种有机硒——L-硒代蛋氨酸（SM）和L-硒甲基硒代半胱氨酸（SMC）的蓄积及抗氧化作用。用10 nM SM或SMC预处理L02细胞24小时，随后暴露于100 nM过氧化氢中。检测细胞活力、凋亡及抗氧化能力，以评估SM和SMC的保护作用。比较有机硒（SM和SMC）和无机硒（亚硒酸钠，SS）在SD大鼠每日补充并随后剥夺时的体内蓄积和抗氧化能力。我们的结果表明，SM或SMC预处理可显著预防凋亡增加和抗氧化能力下降。我们发现，补充有机硒导致肝脏和肾脏中的硒蓄积高于无机硒。喂食有机硒的大鼠肝脏和肾脏组织的抗氧化能力显著提高，且高于SS。总之，本研究表明，有机硒补充剂在促进肝脏和肾脏中硒的蓄积、增强抗氧化能力从而保护细胞免受氧化应激方面更有效。实际应用：本研究比较了亚硒酸钠、L-硒代蛋氨酸和L-硒甲基硒代半胱氨酸在体内外的抗氧化能力。结果表明，有机硒具有更强的抗氧化能力，且在雄性和雌性大鼠中的吸收和转化存在显著差异。我们的结果为膳食补充硒提供了理论指导。
