Microscale Bioseparations Laboratory and Biomedical Engineering Department, Rochester Institute of Technology, Rochester, NY, USA.
Electrophoresis. 2022 Jan;43(1-2):263-287. doi: 10.1002/elps.202100254. Epub 2021 Nov 28.
Miniaturized electrokinetic methods have proven to be robust platforms for the analysis and assessment of intact microorganisms, offering short response times and higher integration than their bench-scale counterparts. The present review article discusses three types of electrokinetic-based methodologies: electromigration or motion-based techniques, electrode-based electrokinetics, and insulator-based electrokinetics. The fundamentals of each type of methodology are discussed and relevant examples from recent reports are examined, to provide the reader with an overview of the state-of-the-art on the latest advancements on the analysis of intact cells and viruses with microscale electrokinetic techniques. The concluding remarks discuss the potential applications and future directions.
微流控电泳技术已被证明是分析和评估完整微生物的强大平台，与传统的台式设备相比，它具有更快的响应时间和更高的集成度。本文综述了三种基于电泳的方法：基于电迁移或运动的技术、基于电极的电泳和基于绝缘体的电泳。讨论了每种方法的基本原理，并研究了最近报道中的相关实例，为读者提供了关于使用微尺度电泳技术分析完整细胞和病毒的最新进展的概述。最后讨论了潜在的应用和未来的发展方向。
