Lapizco-Encinas Blanca H
Microscale Bioseparations Laboratory and Department of Biomedical Engineering, Rochester Institute of Technology, Rochester, New York, USA; email:
Annu Rev Anal Chem (Palo Alto Calif). 2024 Jul;17(1):243-264. doi: 10.1146/annurev-anchem-061622-040810. Epub 2024 Jul 2.
Nonlinear electrokinetic phenomena offer label-free, portable, and robust approaches for particle and cell assessment, including selective enrichment, separation, sorting, and characterization. The field of electrokinetics has evolved substantially since the first separation reports by Arne Tiselius in the 1930s. The last century witnessed major advances in the understanding of the weak-field theory, which supported developments in the use of linear electrophoresis and its adoption as a routine analytical technique. More recently, an improved understanding of the strong-field theory enabled the development of nonlinear electrokinetic techniques such as electrorotation, dielectrophoresis, and nonlinear electrophoresis. This review discusses the operating principles and recent applications of these three nonlinear electrokinetic phenomena for the analysis and manipulation of particles and cells and provides an overview of some of the latest developments in the field of nonlinear electrokinetics.
非线性电动现象为颗粒和细胞评估提供了无标记、便携且强大的方法，包括选择性富集、分离、分选和表征。自20世纪30年代阿恩·蒂塞利乌斯首次发表分离报告以来，电动领域有了很大的发展。上个世纪见证了在弱场理论理解方面的重大进展，这支持了线性电泳的应用发展及其作为常规分析技术的采用。最近，对强场理论的深入理解推动了诸如旋转电泳、介电泳和非线性电泳等非线性电动技术的发展。本文综述了这三种非线性电动现象用于颗粒和细胞分析与操控的工作原理及近期应用，并概述了非线性电动领域的一些最新进展。
