Khan Abdul Latif, Asaf Sajjad, Numan Muhammad, AbdulKareem Noor Mazin, Imran Muhammad, Riethoven Jean-Jack M, Kim Ho-Youn, Al-Harrasi Ahmed, Schachtman Daniel P, Al-Rawahi Ahmed, Lee In-Jung
Department of Engineering Technology, College of Technology, University of Houston, Houston 77479, TX, United States of America; Natural & Medical Sciences Research Center, University of Nizwa, Nizwa 616, Oman.
Natural & Medical Sciences Research Center, University of Nizwa, Nizwa 616, Oman.
Genomics. 2021 Nov;113(6):4337-4351. doi: 10.1016/j.ygeno.2021.11.020. Epub 2021 Nov 16.
Frankincense tree (Boswellia sacra Fluek) has been poorly known on how it responds to tapping and wound-recovery process at molecular levels. Here, we used RNA-sequencing analysis to profile transcriptome of B. sacra after 30 min, 3 h and 6 h of post-tapping. Results showed 5525 differentially expressed genes (DEGs) that were related to terpenoid biosynthesis, phytohormonal regulation, cellular transport, and cell-wall synthesis. Plant-growth-regulators were applied exogenously which showed regulation of endogenous jasmonates and resulted in rapid recovery of cell-wall integrity by significantly up-regulated gene expression of terpenoid biosynthesis (germacrene-D synthase, B-amyrin synthase, and squalene epioxidase-1) and cell-wall synthesis (xyloglucan endotransglucosylase, cellulose synthase-A, and cell-wall hydrolase) compared to control. These findings suggest that tapping immediately activated several cell-developmental and regeneration processes, alongwith defense-induced terpenoid metabolism, to improve the healing process in epidermis. Exogenous growth regulators, especially jasmonic acid, can drastically help tree recovery from tissue degeneration and might help in tree conservation purposes.
乳香树（Boswellia sacra Fluek）在分子水平上对割胶和伤口恢复过程的反应鲜为人知。在此，我们利用RNA测序分析对割胶后30分钟、3小时和6小时的乳香树转录组进行了分析。结果显示，有5525个差异表达基因（DEG）与萜类生物合成、植物激素调节、细胞运输和细胞壁合成有关。外源施加植物生长调节剂，结果表明其对内源茉莉酸有调节作用，并通过与对照相比显著上调萜类生物合成（杜松烯-D合酶、β-香树脂醇合酶和角鲨烯环氧酶-1）和细胞壁合成（木葡聚糖内转糖基酶、纤维素合酶-A和细胞壁水解酶）的基因表达，从而使细胞壁完整性迅速恢复。这些发现表明，割胶立即激活了几个细胞发育和再生过程，以及防御诱导的萜类代谢，以改善表皮的愈合过程。外源生长调节剂，尤其是茉莉酸，能够极大地帮助树木从组织退化中恢复过来，可能有助于树木保护。
