Malve Harshad, More Dipti, More Ashwini
Department of Pharmacology, Vedanta Institute of Medical Sciences, Dahanu, India.
Department of Pediatrics, Lokmanya Tilak Municipal General Hospital and Medical College, Sion, Mumbai, India.
J Ayurveda Integr Med. 2021 Oct-Dec;12(4):682-688. doi: 10.1016/j.jaim.2021.06.021. Epub 2021 Nov 17.
Current pandemic has led us to explore the role of traditional system of medicine to look for formulations that enhance immunity.
The obejctive of this experimental study was to evaluate the immunomodulatory effects of two formulations, Tinospora cordifolia (Tc) and Phyllanthus emblica (Pe) with and without coating of Ocimum sanctum (Os).
After obtaining Institutional Animal Ethics Committee approval, present experimental study was conducted to evaluate the immunomodulatory effects of plant drug formulations against infection induced in mice subjected to major surgical stress. Hemisplenectomy was selected to induce major stress and the procedure for hemisplenectomy was standardized. A model of secondary fungal infection after hemisplenectomy was established followed by the treatment of mice with plant drugs and controls. They were subjected to hemisplenectomy or sham operation and 10 C. albicans were injected intravenously. The therapy continued for next 14 days. Kidneys were isolated to estimate fungal load. Fungal load of the kidneys was estimated on post-operative Day 15.
The test formulations Tc + Pe and Tc + Pe + Os showed significant reduction in the fungal burden of kidneys as compared to hemisplenectomized control group. However, Tc alone exerted better degree of protection as compared to Tc + Pe and Tc + Pe + Os.
The formulations, Tc + Pe and Tc + Pe + Os that were developed on the basis of theoretical concepts were not found to be superior to Tc. Though the individual ingredients have been shown to possess immunnostimulant activities, in combination, Pe and Os blunted the effects of Tc. The basis for this drug interaction needs further exploration. Thus, the current experimental study validates immunomodulatory role of Tc. However, the addition of Pe with bhavana of Os does not lead to any augmentation of immunomodulatory activity of Tc. This study also underlines the need to generate data on Ayurveda formulations to understand the rationality of the multi-ingredient Ayurvedic formulations.
当前的大流行促使我们探索传统医学体系在寻找增强免疫力配方方面的作用。
本实验研究的目的是评估两种配方（匙羹藤（Tc）和余甘子（Pe），有无神圣罗勒（Os）包衣）的免疫调节作用。
在获得机构动物伦理委员会批准后，进行本实验研究，以评估植物药配方对遭受重大手术应激的小鼠感染的免疫调节作用。选择半脾切除术来诱导重大应激，并对半脾切除术的程序进行标准化。建立半脾切除术后继发性真菌感染模型，然后用植物药和对照对小鼠进行治疗。它们接受半脾切除术或假手术，并静脉注射10株白色念珠菌。治疗持续14天。分离肾脏以估计真菌负荷。在术后第15天估计肾脏的真菌负荷。
与半脾切除对照组相比，受试配方Tc + Pe和Tc + Pe + Os显示肾脏真菌负担显著降低。然而，与Tc + Pe和Tc + Pe + Os相比，单独使用Tc具有更好的保护程度。
基于理论概念开发的配方Tc + Pe和Tc + Pe + Os并不优于Tc。尽管各成分已显示具有免疫刺激活性，但Pe和Os联合使用会削弱Tc的效果。这种药物相互作用的基础需要进一步探索。因此，当前的实验研究验证了Tc的免疫调节作用。然而，添加Pe与Os的发酵剂并不会导致Tc免疫调节活性的增强。本研究还强调需要生成关于阿育吠陀配方的数据，以了解多成分阿育吠陀配方的合理性。
