Department of Medical Laboratory, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia.
Department of Optometry, College of Applied Medical Sciences, Qassim University, Buraydah, Saudi Arabia.
J Immunol Res. 2017;2017:1787803. doi: 10.1155/2017/1787803. Epub 2017 Nov 26.
The present study was aimed at determining the activity of aqueous and methanolic extracts of (AETC and METC) against . anti- activity of was determined through the broth dilution and agar well diffusion assays. The immune-stimulating potential of AETC or METC was determined by measuring the cytokine levels in the culture supernatants of treated murine J774 macrophages. Antibacterial activity of AETC or METC was determined by treating infected macrophages and BALB/C mice. The toxicity of AETC or METC was determined by measuring the levels of liver inflammation markers aspartate transaminase (AST) and alanine transaminase (ALT) and antioxidant enzymes. Macrophages treated with AETC or METC secreted greater levels of IFN-, TNF-, and IL-1. METC showed greater activity against infection in macrophages and mice as well. Treatment with METC resulted in increased survival and reduced bacterial load in -infected mice. Moreover, METC or AETC treatment reduced the liver inflammation and rescued the levels of antioxidant enzymes in -infected mice. The results of the present study suggest that the use of may act as a double-edged sword in combating salmonellosis.
本研究旨在确定 （AETC 和 METC）的水提物和醇提物对 的活性。通过肉汤稀释法和琼脂孔扩散法测定 的抗活性。通过测量经处理的鼠 J774 巨噬细胞培养上清液中的细胞因子水平来确定 AETC 或 METC 的免疫刺激潜力。通过处理感染的巨噬细胞和 BALB/C 小鼠来测定 AETC 或 METC 的抗菌活性。通过测量肝炎症标志物天冬氨酸转氨酶（AST）和丙氨酸转氨酶（ALT）和抗氧化酶的水平来确定 AETC 或 METC 的毒性。用 AETC 或 METC 处理的巨噬细胞分泌更高水平的 IFN-、TNF-和 IL-1。METC 对巨噬细胞和小鼠中的 感染也表现出更强的活性。METC 治疗可提高感染小鼠的存活率并降低细菌负荷。此外，METC 或 AETC 治疗可减轻感染小鼠的肝炎症并恢复抗氧化酶水平。本研究的结果表明，使用 可能在治疗沙门氏菌病方面具有双重作用。
