抗乙酰胆碱抗体与重症肌无力的发病机制

Anti-acetylcholine antibodies and the pathogenesis of myasthenia gravis.

作者信息

Souan M L, Geffard M, Vieillemaringe J, Lebrun-Grandie P, Orgogozo J M

机构信息

Laboratoire de Neuroimmunologie, IBCN-CNRS, Bordeaux, France.

出版信息

Ann N Y Acad Sci. 1987;505:423-38. doi: 10.1111/j.1749-6632.1987.tb51313.x.

DOI:10.1111/j.1749-6632.1987.tb51313.x

PMID:3479933

Abstract

Using an ELISA system, antibodies recognizing conjugated acetylcholine (ACh) were detected in sera of patients suffering from myasthenia gravis. The mean antibody level was three times higher in sera from myasthenic than from control patients. No correlation was found between anti-ACh antibody levels and anti-ACh receptor (AChR) titer. Also, the anti-ACh antibody titers were independent of sex and age of patients. Competition experiments demonstrated that the most immunoreactive compounds were choline-glutaryl-bovine serum albumin (BSA) and choline-succinyl-BSA. Antibodies present in the sera of myasthenic patients recognized an antigenic determinant mimicking conjugated ACh. The antibody affinity and specificity were sufficiently high for the detection of ACh in locust brain.

摘要

使用酶联免疫吸附测定（ELISA）系统，在重症肌无力患者的血清中检测到了识别结合型乙酰胆碱（ACh）的抗体。重症肌无力患者血清中的平均抗体水平是对照患者血清的三倍。未发现抗ACh抗体水平与抗ACh受体（AChR）滴度之间存在相关性。此外，抗ACh抗体滴度与患者的性别和年龄无关。竞争实验表明，免疫反应性最强的化合物是胆碱-戊二酰-牛血清白蛋白（BSA）和胆碱-琥珀酰-BSA。重症肌无力患者血清中存在的抗体识别一种模拟结合型ACh的抗原决定簇。该抗体的亲和力和特异性足以用于检测蝗虫脑中的ACh。