Department of Cell and Systems Biology, University of Toronto, Toronto, ON M5S 3B2, Canada.
Agriculture and Agri-Food Canada, Carleton University, Ottawa, ON K1A 0C6, Canada.
Proc Natl Acad Sci U S A. 2021 Nov 23;118(47). doi: 10.1073/pnas.2116570118.
Pathogenic effector proteins use a variety of enzymatic activities to manipulate host cellular proteins and favor the infection process. However, these perturbations can be sensed by nucleotide-binding leucine-rich-repeat (NLR) proteins to activate effector-triggered immunity (ETI). Here we have identified a small molecule (Zaractin) that mimics the immune eliciting activity of the type III secreted effector (T3SE) HopF1r and show that both HopF1r and Zaractin activate the same NLR-mediated immune pathway in Our results demonstrate that the ETI-inducing action of pathogenic effectors can be harnessed to identify synthetic activators of the eukaryotic immune system.
病原效应蛋白利用多种酶活性来操纵宿主细胞蛋白，从而有利于感染过程。然而，这些干扰可以被核苷酸结合富含亮氨酸重复（NLR）蛋白感知，从而激活效应子触发的免疫（ETI）。在这里，我们鉴定出一种小分子（Zaractin），它模拟了 III 型分泌效应子（T3SE）HopF1r 的免疫激发活性，并表明 HopF1r 和 Zaractin 都在 中激活相同的 NLR 介导的免疫途径。我们的结果表明，可以利用病原效应蛋白诱导 ET 的作用来鉴定真核免疫系统的合成激活剂。
