单个哺乳动物细胞中游离钙的水母发光蛋白测量法。
Aequorin measurements of free calcium in single mammalian cells.
作者信息
Cobbold P H, Cuthbertson K S, Goyns M H, Rice V
出版信息
J Cell Sci. 1983 May;61:123-36. doi: 10.1242/jcs.61.1.123.
PMID:6350328
Abstract
The calcium-triggered photoprotein aequorin has been used as a sensitive indicator of cytoplasmic free calcium in cells of relatively large size. We describe here several novel modifications to the technique that make it possible to detect resting levels of free calcium in single mammalian oocytes, fibroblasts and granulosa cells. Concentrations of free calcium as low as 1 X 10(-7) M have been measured in single fibroblasts, corresponding to a detection limit of 10(-18)mol.
摘要
钙触发的光蛋白水母发光蛋白已被用作相对较大细胞中细胞质游离钙的敏感指示剂。我们在此描述了对该技术的几种新改进，这些改进使得能够检测单个哺乳动物卵母细胞、成纤维细胞和颗粒细胞中游离钙的静息水平。在单个成纤维细胞中已检测到低至1×10⁻⁷M的游离钙浓度，对应于10⁻¹⁸摩尔的检测限。
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