School of Food and Biological Engineering, Jiangsu University, Zhenjiang, China; Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing 210014, China; Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.
Institute of Veterinary Medicine, Jiangsu Academy of Agricultural Sciences, Key Laboratory of Veterinary Biological Engineering and Technology Ministry of Agriculture, Nanjing 210014, China; School of Materials Science & Engineering, Jiangsu University, Zhenjiang, China; Jiangsu Key Laboratory for Food Quality and Safety-State Key Laboratory Cultivation Base of Ministry of Science and Technology, Nanjing 210014, China.
Int J Biol Macromol. 2022 Jan 1;194:435-444. doi: 10.1016/j.ijbiomac.2021.11.085. Epub 2021 Nov 19.
In this work, an edible cellulose-based antibacterial material was prepared by cross-linking α-cellulose and kanamycin sulfate via glutaraldehyde to form kanamycin sulfate-glutaraldehyde-cellulose. Fourier transform infrared spectroscopy, X-ray photoelectron spectroscopy and X-ray diffraction results indicated that the kanamycin sulfate molecule was cross-linked with the molecular chain of cellulose. The optimal mass ratio of kanamycin sulfate to α-cellulose was 1:100 and the degree of substitution reached 1.11%. The optimal kanamycin sulfate-glutaraldehyde-cellulose material showed an excellent inhabitation against both Gram-positive and Gram-negative bacteria. Meantime, the optimal kanamycin sulfate-glutaraldehyde-cellulose had a marked resistance to gastric acid and had low cell cytotoxicity. To promote the application of the kanamycin sulfate-glutaraldehyde-cellulose material, the porous microspheres were prepared via the sol-gel method. The particle size of the homogeneous porous microspheres is mainly distributed between 1.5 and 2.0 μm. Therefore, the kanamycin sulfate-glutaraldehyde-cellulose described herein is a potential edible, eco-friendly, potent, stable, inexpensive, and antibacterial carrier material for delivering drugs, proteins, or vaccines.
在这项工作中，通过戊二醛交联α-纤维素和硫酸卡那霉素，制备了一种可食用的纤维素基抗菌材料硫酸卡那霉素-戊二醛-纤维素。傅里叶变换红外光谱、X 射线光电子能谱和 X 射线衍射结果表明，硫酸卡那霉素分子与纤维素分子链发生了交联。硫酸卡那霉素与α-纤维素的最佳质量比为 1:100，取代度达到 1.11%。最佳的硫酸卡那霉素-戊二醛-纤维素材料对革兰氏阳性菌和革兰氏阴性菌均具有优异的抑制作用。同时，最佳的硫酸卡那霉素-戊二醛-纤维素具有良好的抗胃酸能力，细胞毒性较低。为了促进硫酸卡那霉素-戊二醛-纤维素材料的应用，采用溶胶-凝胶法制备了多孔微球。均匀多孔微球的粒径主要分布在 1.5-2.0μm 之间。因此，本文所述的硫酸卡那霉素-戊二醛-纤维素是一种具有潜在应用价值的可食用、环保、高效、稳定、廉价的抗菌药物载体材料，可用于输送药物、蛋白质或疫苗。
