Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
Department of Biochemistry, Faculty of Science, Ain Shams University, Cairo, Egypt.
Tissue Cell. 2022 Feb;74:101679. doi: 10.1016/j.tice.2021.101679. Epub 2021 Nov 18.
It is known that SARS-CoV-2 mostly infects the respiratory system causing pneumonia; although it can also affect the gastrointestinal tract (GIT), which covered with a bi-layer of mucus rich in glycosylated proteins that terminated by sialic acid. Therefore; this study aimed to evaluate serum total sialic acid (TSA) in moderate COVID-19 patients with and without GIT manifestations.
A total of 161 moderate COVID-19 patients without and with GIT manifestations and 50 controls were enrolled into our study. Serum electrolytes levels were measured by using colorimetric or turbidmetric commercial assay kits, while the level of serum TSA was measured by using a commercial ELISA kit.
Our results showed that serum TSA level was highly significantly increased in moderate COVID-19 patients with GIT manifestations (81.43 ± 8.91) when compared with controls (61.24 ± 6.41) or even moderate COVID-19 patients without GIT manifestations (69.46 ± 7.03). ROC curve analysis showed that AUC for TSA is 0.84 with 76.2 % sensitivity and 73.7 % specificity in discrimination between moderate COVID-19 patients with and without GIT manifestations. Serum potassium and sodium levels were highly significantly decreased in moderate COVID-19 patients with GIT manifestations when compared with controls or even moderate COVID-19 patients without GIT manifestations; while serum calcium level was found to be significantly decreased in moderate COVID-19 patients with GIT manifestations when compared with controls.
Finally, we can conclude that SA plays a crucial role in the pathogenesis of GIT complications associated with COVID-19 and could be a potential biomarker for the COVID-19 gastrointestinal complications.
已知 SARS-CoV-2 主要感染呼吸系统引起肺炎；尽管它也可能影响胃肠道（GIT），GIT 覆盖着富含糖基化蛋白的双层黏液，其末端为唾液酸。因此；本研究旨在评估有和无 GIT 表现的中度 COVID-19 患者的血清总唾液酸（TSA）。
共纳入 161 例无 GIT 表现和有 GIT 表现的中度 COVID-19 患者及 50 名对照者。使用比色法或比浊法商业检测试剂盒测定血清电解质水平，使用商业 ELISA 试剂盒测定血清 TSA 水平。
我们的结果表明，与对照组（61.24±6.41）或甚至无 GIT 表现的中度 COVID-19 患者（69.46±7.03）相比，有 GIT 表现的中度 COVID-19 患者的血清 TSA 水平显著升高（81.43±8.91）。ROC 曲线分析显示，TSA 的 AUC 为 0.84，在区分有和无 GIT 表现的中度 COVID-19 患者时具有 76.2%的敏感性和 73.7%的特异性。与对照组或甚至无 GIT 表现的中度 COVID-19 患者相比，有 GIT 表现的中度 COVID-19 患者的血清钾和钠水平显著降低；而有 GIT 表现的中度 COVID-19 患者的血清钙水平则明显降低。
最后，我们可以得出结论，SA 在与 COVID-19 相关的 GIT 并发症发病机制中起关键作用，并且可能是 COVID-19 胃肠道并发症的潜在生物标志物。
