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含硼化合物对基础状态下免疫应答细胞和抗体的影响的多样性。

Diversity of effects induced by boron-containing compounds on immune response cells and on antibodies in basal state.

机构信息

Laboratorio de Inmunidad de Mucosas, Sección de Estudios de Posgrado e Investigación, Escuela Superior de Medicina (ESM) del Instituto Politécnico Nacional (IPN), Plan de San Luis y Díaz Mirón s/n, 11340 Mexico City, Mexico.

Academias de Fisiología, Bioquímica y Sección de Estudios de Posgrado e Investigación del IPN, Plan de San Luis y Díaz Mirón s/n, 11340 Mexico City, Mexico.

出版信息

J Trace Elem Med Biol. 2022 Jan;69:126901. doi: 10.1016/j.jtemb.2021.126901. Epub 2021 Nov 15.

DOI:10.1016/j.jtemb.2021.126901

PMID:34801850

Abstract

BACKGROUND

It has been reported that boron induces changes in the immune response, including in inflammatory processes. Recently, the effect of boric acid has been documented on the differentiation of lymphocyte clusters in mice and rats. However, the differences among boron-containing compounds (BCC) have been poorly explored.

METHODS

In this study, we analyzed the effects after oral administration of boric acid (BOR), methylboronic (MET), 3-thyenylboronic (3TB), 4-hydroxymethyl-phenylboronic (4MP) and 4-methanesulfonyl-phenylboronic (4SP) acids on the populations of lymphocytes from spleen and Peyer's patch (PP) as well as on antibodies. Groups of six male BALB/c were orally treated with 4.6 mg/kg of body weight with BOR, MET, 3TB, 4MP, and 4SP/daily for 10 days or vehicle (VEH) as a control group. After euthanasia, the spleen and small intestine were dissected. We conducted flow cytometry assays to assess B, CD3 T, CD4 T, and CD8 T cells. Levels of IgG and IgM in serum, and IgA in intestinal fluid samples were analyzed by enzyme immunoassay.

RESULTS

In particular, we observed the effects of the administration of boronic acids on the number of lymphocytes; these changes were more notable in spleen than in PP. We found different profiles for each boron-containing compound, that is BOR induced an increase in the percentage of CD8 T and CD19/IgA cells in spleen, but a decrease in CD8 T and B220/CD19 cells in PP. Meanwhile MET induced a decrease of CD4 T in spleen, but induced an increase of CD4 T cells and a decrease in the number of CD8 T cells in PP. Boronic acids with an aromatic ring moiety induced changes in serum immunoglobulins levels, while 3TB acid induced a notable increase in S-IgA.

CONCLUSIONS

Effects in lymphocyte populations and antibodies are different for each tested compound. These results highlight the establishment of the necessary structure-activity relationship for BCC as immunomodulatory drugs. This is relevant in the biomedical field due to their attractiveness for selecting compounds to develop therapeutic tools.

摘要

背景

据报道，硼能诱导免疫反应的变化，包括炎症过程。最近，硼酸对小鼠和大鼠淋巴细胞簇分化的影响已被记录下来。然而，含硼化合物（BCC）之间的差异尚未得到充分探索。

方法

在这项研究中，我们分析了经口给予硼酸（BOR）、甲基硼酸（MET）、3-噻吩硼酸（3TB）、4-羟甲基苯硼酸（4MP）和 4-甲磺酰基苯硼酸（4SP）后对脾脏和派尔氏斑（PP）淋巴细胞群以及抗体的影响。6 只雄性 BALB/c 被分为 6 组，每天经口给予 4.6mg/kg 的 BOR、MET、3TB、4MP 和 4SP，或给予载体（VEH）作为对照组，共 10 天。安乐死后，解剖脾脏和小肠。我们通过流式细胞术检测来评估 B、CD3 T、CD4 T 和 CD8 T 细胞。通过酶免疫测定法分析血清 IgG 和 IgM 水平以及肠液样本中 IgA 的水平。

结果

特别地，我们观察了硼酸盐给药对淋巴细胞数量的影响；这些变化在脾脏中比在 PP 中更为明显。我们发现每种含硼化合物都有不同的特征，即 BOR 诱导脾脏中 CD8 T 和 CD19/IgA 细胞的百分比增加，但 PP 中 CD8 T 和 B220/CD19 细胞的百分比减少。同时，MET 诱导脾脏中 CD4 T 的减少，但诱导 PP 中 CD4 T 细胞的增加和 CD8 T 细胞的减少。具有芳环部分的硼酸盐诱导血清免疫球蛋白水平的变化，而 3TB 酸诱导 S-IgA 的显著增加。