Department of Orthopaedic Surgery, Second Affiliated Hospital and Zhejiang University-University of Edinburgh Institute and School of Basic Medicine, Zhejiang University School of Medicine, Hangzhou, China; Dr. Li Dak Sum & Yip Yio Chin Center for Stem Cells and Regenerative Medicine, Zhejiang University School of Medicine, Hangzhou, China; Department of Orthopedics, Hunan Cancer Hospital and The Affiliated Cancer Hospital of Xiangya School of Medicine, Central South University, Changsha 410013, China.
Health Care Department for Women, The Maternal And Child Health Hospital Of Hunan Province, Changsha 410008, China.
Colloids Surf B Biointerfaces. 2022 Jan;209(Pt 1):112214. doi: 10.1016/j.colsurfb.2021.112214. Epub 2021 Nov 16.
Biodegradable and absorbable sutures have been widely used in surgical procedures. However, for the repair of ligament and tendon injures, the biodegradable suture cannot provide sufficient mechanical support to close the wound for a long period of time which is important to completely heal the tissue. Herein, we develop a simple method that makes a surface coating to prolong the degradation of the suture in vivo. Polylactic acid (PLLA) and Polycaprolactone (PCL) were successfully coated to a commercial degradable polydioxanone (PDO) suture in this study, which was confirmed by Fourier transform infrared spectra (FTIR). Scanning electron microscopy (SEM) was used to observe the smooth surface of the coated sutures. Moreover, live/dead assay of human fibroblasts after co-culturing with the modified/unmodified sutures showed fairly good cellular activity. In vivo study demonstrates the degradation properties of sutures were significantly changed after the surface coating. The raw suture exhibited the fastest degradation in 12 weeks, showing significantly decline in mechanical strength. Interestingly, the PCL-coated suture was able to maintain more than 20% of its original tensile strength after 12 weeks' implantation. In addition, in vivo results of PCL-coated sutures also showed less inflammatory cell infiltration and less surface inflammation. These findings indicate the one step suture-coating method could be feasibly for the development of clinical equipment.
可生物降解和可吸收缝线已广泛应用于外科手术中。然而，对于韧带和肌腱损伤的修复，可生物降解缝线不能提供足够的机械支撑来长时间闭合伤口，这对于组织完全愈合非常重要。在此，我们开发了一种简单的方法，通过表面涂层来延长缝线在体内的降解。本研究成功地将聚乳酸（PLLA）和聚己内酯（PCL）涂覆到一种商业可降解聚二氧环己酮（PDO）缝线，这一点通过傅里叶变换红外光谱（FTIR）得到了证实。扫描电子显微镜（SEM）用于观察涂层缝线的光滑表面。此外，与人成纤维细胞共培养后，用改良/未改良缝线进行的死活检测显示出相当好的细胞活性。体内研究表明，表面涂层后缝线的降解特性发生了显著变化。原始缝线在 12 周内表现出最快的降解，其机械强度明显下降。有趣的是，PCL 涂层缝线在植入 12 周后仍能保持其原始拉伸强度的 20%以上。此外，PCL 涂层缝线的体内结果还显示出炎症细胞浸润和表面炎症较少。这些发现表明，一步法缝线涂层方法可能可行，可用于开发临床设备。
