Laboratory of Lymphocyte Dynamics, The Rockefeller University, New York, NY, USA.
Methods Mol Biol. 2022;2380:15-27. doi: 10.1007/978-1-0716-1736-6_2.
Germinal centers (GCs) are microanatomical structures in secondary lymphoid organs where B cells undergo affinity maturation for antigen during the course of an immune response. This process is driven by a subset of T cells termed T follicular helper cells (Tfh) that through a multistep process gain access to the GC niche within the B cell follicle. This protocol details how to study Tfh behavior in vivo, on a single cell level, using two-photon intravital microscopy of the murine popliteal lymph node.
生发中心（GCs）是次级淋巴器官中的微观解剖结构，B 细胞在免疫应答过程中针对抗原进行亲和力成熟。这一过程由一类称为滤泡辅助性 T 细胞（Tfh）的 T 细胞亚群驱动，它们通过多步过程进入 B 细胞滤泡内的 GC 龛位。本方案详细介绍了如何使用活体双光子显微镜对鼠腘淋巴结进行研究，在单细胞水平上研究 Tfh 行为。
