Division of Rheumatology, Inflammation, Immunology, Brigham and Women's Hospital and Harvard Medical School, Boston, MA, USA.
Methods Mol Biol. 2022;2380:59-76. doi: 10.1007/978-1-0716-1736-6_6.
T peripheral helper (Tph) cells represent a T cell population that stimulates B cell responses within peripheral tissues. Like T follicular helper (Tfh) cells, PD-1 CXCR5 CD4 Tph cells produce IL-21 and CXCL13, yet these cells differ from Tfh cells in expression of both transcription factors and chemokine receptors. Originally identified in rheumatoid arthritis synovium, Tph cells are expanded in multiple autoimmune diseases. Tph cells can be identified and distinguished from Tfh cells by both flow cytometry and mass cytometry. Here, we describe detailed methods to identify Tph cells and characterize associated phenotypic features and functions. These protocols and additional notes provide a guide to identify and explore the roles of Tph cells in various human diseases.
辅助性 T 细胞（Tph）代表一类刺激外周组织中 B 细胞反应的 T 细胞群体。与滤泡辅助性 T 细胞（Tfh）类似，PD-1+CXCR5+CD4+Tph 细胞也会产生 IL-21 和 CXCL13，但这些细胞在转录因子和趋化因子受体的表达上与 Tfh 细胞存在差异。Tph 细胞最初在类风湿关节炎滑膜中被鉴定出来，在多种自身免疫性疾病中均有扩增。Tph 细胞可通过流式细胞术和液质联用技术进行鉴定和区分。本文详细介绍了鉴定 Tph 细胞并对其相关表型特征和功能进行分析的方法。这些方案和补充说明为鉴定和研究 Tph 细胞在各种人类疾病中的作用提供了指导。
