Laboratory of Dynamic Immunobiology, Institute for Immunology, Beijing, China.
Department of Basic Medical Sciences, School of Medicine, Tsinghua-Peking Center for Life Sciences, Tsinghua University, Beijing, China.
Methods Mol Biol. 2022;2380:99-109. doi: 10.1007/978-1-0716-1736-6_9.
Follicular T-helper cells play a key role in orchestrating the germinal center response. Cellular dynamics revealed by intravital imaging have helped deepen our understanding of how follicular T-helper cells develop and function in vivo. Here we describe routine methods to image T-cell dynamics during germinal center responses. Methods for quantitative analysis of imaging data are detailed using published examples.
滤泡辅助性 T 细胞在协调生发中心反应中发挥关键作用。活体成像揭示的细胞动态变化有助于加深我们对滤泡辅助性 T 细胞在体内如何发育和发挥功能的理解。本文描述了在生发中心反应期间成像 T 细胞动态变化的常规方法。并使用已发表的示例详细介绍了成像数据的定量分析方法。
