Toulouse Institute for Infectious and Inflammatory Diseases (Infinity), Institut National de la Santé et de la Recherche (INSERM), Centre National de la Recherche Scientifique (CNRS), Université Toulpouse III Paul-Sabatier, Toulouse, France.
Methods Mol Biol. 2022;2380:141-147. doi: 10.1007/978-1-0716-1736-6_12.
Characterization and counting of the different immune cell subpopulations are largely used in order to predict the quality of vaccination or the progression of diseases. As such, flow cytometry is a valuable technology to perform an exact cartography of the immune cell subsets. In the context of B-cell responses, specialized structures emerge in B-follicles of second lymphoid organs where B-cells "undergo maturation processes under the guidance of specific T-cells, follicular helper T-cells, and follicular regulatory T-cells. Thus, tracking these cell types is of high interest, especially in the context of protein vaccination. In this purpose, we describe here, how we can track antigen-specific follicular helper T-cells and follicular regulatory T-cells by flow cytometry after protein vaccination in nonmodified wild-type mice, which ultimately provides a comprehensive way to better understand the function of these particular cells in vivo.
为了预测疫苗接种质量或疾病进展，对不同免疫细胞亚群进行鉴定和计数是非常重要的。因此，流式细胞术是一种对免疫细胞亚群进行精确绘图的有价值的技术。在 B 细胞反应的背景下，二级淋巴器官的 B 滤泡中出现了专门的结构，B 细胞在特异性 T 细胞、滤泡辅助 T 细胞和滤泡调节性 T 细胞的指导下“经历成熟过程”。因此，跟踪这些细胞类型非常重要，特别是在蛋白质疫苗接种的情况下。在本研究中，我们描述了如何在未经修饰的野生型小鼠中通过流式细胞术在蛋白质疫苗接种后跟踪抗原特异性滤泡辅助 T 细胞和滤泡调节性 T 细胞，这最终为更好地理解这些特定细胞在体内的功能提供了一种全面的方法。
