补体 C4-A 和纤溶酶原作为预测甲状腺乳头状癌的潜在生物标志物。
Complement C4-A and Plasminogen as Potential Biomarkers for Prediction of Papillary Thyroid Carcinoma.
机构信息
Department of Thyroid & Parathyroid Surgery Center, West China Hospital, Sichuan University, Chengdu, China.
Laboratory of Thyroid and Parathyroid Disease, Frontiers Science Center for Disease-related Molecular Network, West China Hospital, Sichuan University, Chengdu, China.
出版信息
Front Endocrinol (Lausanne). 2021 Nov 5;12:737638. doi: 10.3389/fendo.2021.737638. eCollection 2021.
BACKGROUND
Early diagnosis and therapy of papillary thyroid carcinoma (PTC) is essential for reducing recurrence and improving the long-term survival. In this study, we aimed to investigate the proteome profile of plasma and screen unique proteins which could be used as a biomarker for predicting PTC.
METHODS
Serum samples were collected from 29 PTC patients and 29 nodular goiter (NG) patients. Five PTC serum samples and five NG serum samples were selected for proteome profiles by proteomics. Eight proteins in PTC and NG serum samples were selected for confirmation by enzyme-linked immunosorbent assay analysis. Receiver operating characteristic curves was used to evaluate the diagnostic value of potential biomarkers.
RESULTS
Complement C4-A (C4A) and plasminogen (PLG) were significantly lower in serum samples of PTC patients compared with NG patients. C4A was observed to have excellent diagnostic accuracy for PTC, with a sensitivity of 91.67% and specificity of 83.33%. The diagnostic value of PLG for PTC was demonstrated by a sensitivity at 87.50% and specificity at 75.00%. The AUC for C4A and PLG was 0.97 ± 0.02 and 0.89 ± 0.05.
CONCLUSION
C4A and PLG appeared to be excellent potential biomarkers for the prediction of PTC.
背景
早期诊断和治疗甲状腺乳头状癌（PTC）对于降低复发率和提高长期生存率至关重要。本研究旨在探讨血浆的蛋白质组谱，并筛选出可作为预测 PTC 的生物标志物的独特蛋白。
方法
收集了 29 例 PTC 患者和 29 例结节性甲状腺肿（NG）患者的血清样本。通过蛋白质组学对 5 例 PTC 血清样本和 5 例 NG 血清样本进行蛋白质组谱分析。通过酶联免疫吸附分析对 PTC 和 NG 血清样本中的 8 种蛋白质进行确认。使用受试者工作特征曲线评估潜在生物标志物的诊断价值。
结果
与 NG 患者相比，PTC 患者血清样本中的补体 C4-A（C4A）和纤溶酶原（PLG）明显降低。C4A 对 PTC 具有出色的诊断准确性，敏感性为 91.67%，特异性为 83.33%。PLG 对 PTC 的诊断价值通过 87.50%的敏感性和 75.00%的特异性得到证明。C4A 和 PLG 的 AUC 分别为 0.97±0.02 和 0.89±0.05。
结论
C4A 和 PLG 似乎是预测 PTC 的优秀潜在生物标志物。
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