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[具体物种名称]中的小RNA分析为应激适应性提供了见解。

Small RNA Profiling in Provides Insights Into Stress Adaptability.

作者信息

Chen Yingyu, Zhai Wenjun, Zhang Kailun, Liu Han, Zhu Tingting, Su Li, Bermudez Luiz, Chen Huanchun, Guo Aizhen

机构信息

State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, China.

National Animal Tuberculosis Para-Reference Laboratory (Wuhan) of Ministry of Agriculture and Rural Affairs, Huazhong Agricultural University, Wuhan, China.

出版信息

Front Microbiol. 2021 Nov 4;12:752537. doi: 10.3389/fmicb.2021.752537. eCollection 2021.

DOI:10.3389/fmicb.2021.752537
PMID:34803973
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8600241/
Abstract

encounter a number of environmental changes during infection and respond using different mechanisms. Small RNA (sRNA) is a post-transcriptionally regulatory system for gene functions and has been investigated in many other bacteria. This study used and Bacillus Calmette-Guérin (BCG) infection models and sequenced whole bacterial RNAs before and after host cell infection. A comparison of differentially expressed sRNAs using Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) and target prediction was carried out. Six pathogenically relevant stress conditions, growth rate, and morphology were used to screen and identify sRNAs. From these data, a subset of sRNAs was differentially expressed in multiple infection groups and stress conditions. Many were found associated with lipid metabolism. Among them, ncBCG427 was significantly downregulated when BCG entered into macrophages and was associated with increased biofilm formation. The reduction of virulence possibility depends on regulating lipid metabolism.

摘要

在感染过程中会遇到许多环境变化，并通过不同机制做出反应。小RNA（sRNA）是一种基因功能的转录后调控系统，已在许多其他细菌中进行了研究。本研究使用了和卡介苗（BCG）感染模型，并对宿主细胞感染前后的全细菌RNA进行了测序。使用基因本体论（GO）和京都基因与基因组百科全书（KEGG）对差异表达的sRNA进行了比较，并进行了靶标预测。使用六种与致病性相关的应激条件、生长速率和形态来筛选和鉴定sRNA。从这些数据中，一部分sRNA在多个感染组和应激条件下差异表达。许多sRNA被发现与脂质代谢有关。其中，ncBCG427在卡介苗进入巨噬细胞时显著下调，并与生物膜形成增加有关。毒力降低的可能性取决于对脂质代谢的调节。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d93/8600241/403b75809364/fmicb-12-752537-g013.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d93/8600241/bf8e1ef35803/fmicb-12-752537-g004.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d93/8600241/11f77cb48b78/fmicb-12-752537-g007.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d93/8600241/70ec116e127d/fmicb-12-752537-g008.jpg
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https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d93/8600241/ae969d5711ad/fmicb-12-752537-g010.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d93/8600241/71dda0da0143/fmicb-12-752537-g011.jpg
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