Appl Opt. 2021 Nov 10;60(32):10214-10220. doi: 10.1364/AO.437640.
This works presents a reconstruction algorithm to recover the complex object information for an off-axis digital holographic microscope (DHM) operating in the telecentric regimen. We introduce an automatic and fast method to minimize a cost function that finds the best numerical conjugated reference beam to compensate the filtered object information, eliminating any undesired phase perturbation due to the tilt between the reference and object waves. The novelties of the proposed approach, to the best of our knowledge, are a precise estimation of the interference angle between the object and reference waves, reconstructed phase images without phase perturbations, and reduced processing time. The method has been validated using a manufactured phase target and biological samples.
本文提出了一种重建算法，用于恢复离轴数字全息显微镜（DHM）在远心模式下的复物体信息。我们引入了一种自动且快速的方法来最小化代价函数，该函数找到最佳的数值共轭参考光束来补偿滤波后的物体信息，消除由于参考和物体波之间的倾斜而产生的任何不需要的相位扰动。据我们所知，该方法的新颖之处在于精确估计物体和参考波之间的干涉角、重建相位图像而无相位扰动，以及缩短处理时间。该方法已使用制造的相位目标和生物样本进行了验证。
