Mattern I E, Unkles S, Kinghorn J R, Pouwels P H, van den Hondel C A
Medical Biological Laboratory TNO, Rijswijk, The Netherlands.
Mol Gen Genet. 1987 Dec;210(3):460-1. doi: 10.1007/BF00327197.
A transformation system for Aspergillus oryzae based on the orotidine-5'-phosphate decarboxylase gene (pyrG) was developed. Transformation frequencies of up to 16 transformants per microgram of DNA were obtained with the vector pAB4-1, which carries the pyrG gene of A. niger. Southern blotting analysis showed that vector DNA sequences were integrated into the chromosomal DNA, in various copy numbers and presumably at different sites. Efficient cotransformation of an unselectable gene was also shown. Under the conditions used no transformants were obtained with the equivalent pyr4 gene of Neurospora crassa.
基于乳清苷-5'-磷酸脱羧酶基因（pyrG）开发了米曲霉转化系统。使用携带黑曲霉pyrG基因的载体pAB4-1，获得了每微克DNA高达16个转化体的转化频率。Southern印迹分析表明，载体DNA序列以不同的拷贝数整合到染色体DNA中，推测整合位点也不同。同时也证明了不可选择基因的高效共转化。在所使用的条件下，用粗糙脉孢菌的等效pyr4基因未获得转化体。
