Nuralın Levent, Gürü Metin
Department of Chemical Engineering, Engineering Faculty of Gazi University, Ankara, Turkey.
Food Anal Methods. 2022;15(4):877-889. doi: 10.1007/s12161-021-02136-8. Epub 2021 Nov 17.
Recently, research studies on nutraceutically important polyphenolic substances have attracted intensive attention. is an important source of polyphenolic compounds and is often used in traditional medicine. In this study, the extraction of rutin and apigenin rich oil from fruits was evaluated by supercritical carbon dioxide (Sc-CO) extraction method with and without co-solvent. As valuable antioxidants, rutin and apigenin content of extracts were analyzed by HPLC, and their amounts were maximized via parametric optimization. The rutin compound studied in this research has the potential to be a drug against the COVID-19 virus. The operating conditions were considered in the range of 35-70 °C temperatures, 140-240 bar of pressures, 0.35-1.00 mm of mean particle sizes, 3-7 l/min of CO flow rates, and 0-8% w/w co-solvent. As a result of Sc-CO extractions, the amounts of rutin and apigenin were found as 173 ± 14.97 µg/g and 2.91 ± 0.11 µg/g, respectively, with the 8% (w/w) co-solvent addition. The amounts of rutin and apigenin obtained by Soxhlet extractions were found as 208.81 ± 8.48 µg/g and 6.55 ± 0.21 µg/g, respectively. When the Sc-CO method was compared with the Soxhlet method, it was seen that the fast and eco-friendly Sc-CO method was an ideal extraction method by providing 76.89% rutin and 44.53% apigenin recoveries. As a result of this study, the maximum extraction conditions for rutin and apigenin were obtained as 160 bar, 40 °C, 0.35 mm particle size, 3 l/min CO, 8% w/w co-solvent ratio, and 120 min extraction period.
The online version contains supplementary material available at 10.1007/s12161-021-02136-8.
最近，对具有营养保健重要性的多酚类物质的研究吸引了广泛关注。[具体物质]是多酚类化合物的重要来源，常用于传统医学。在本研究中，采用超临界二氧化碳（Sc-CO₂）萃取法，在有无共溶剂的情况下，对从[具体水果]果实中提取富含芦丁和芹菜素的油进行了评估。作为有价值的抗氧化剂，通过高效液相色谱法分析提取物中的芦丁和芹菜素含量，并通过参数优化使其含量最大化。本研究中所研究的芦丁化合物有潜力成为一种抗新冠病毒的药物。操作条件考虑的范围为温度35 - 70°C、压力140 - 240巴、平均粒径0.35 - 1.00毫米、CO₂流速3 - 7升/分钟以及共溶剂0 - 8%（w/w）。超临界二氧化碳萃取的结果是，添加8%（w/w）共溶剂时，芦丁和芹菜素的含量分别为173±14.97微克/克和2.91±0.11微克/克。索氏提取法得到的芦丁和芹菜素含量分别为208.81±8.48微克/克和6.55±0.21微克/克。将超临界二氧化碳方法与索氏方法进行比较时发现，快速且环保的超临界二氧化碳方法通过提供76.89%的芦丁回收率和44.53%的芹菜素回收率，是一种理想的提取方法。本研究的结果是，芦丁和芹菜素的最大提取条件为160巴、40°C、粒径0.35毫米、CO₂流速3升/分钟、共溶剂比例8%（w/w）以及提取时间120分钟。
在线版本包含可在10.1007/s12161-021-02136-8获取的补充材料。
