Department of Pharmacognosy, Faculty of Pharmacy and Biochemistry, University of Zagreb, Marulićev trg 20, 10 000 Zagreb, Croatia.
Department of Food Chemistry, Faculty of Pharmacy and Biochemistry, University of Zagreb, Domagojeva 1, 10 000 Zagreb, Croatia.
Molecules. 2019 Oct 8;24(19):3613. doi: 10.3390/molecules24193613.
is rich in berberine, an isoquinoline alkaloid, with antidiabetic activity, often used topically for skin-related problems. The aim of this work was to develop a "green" method for berberine extraction using mixtures of water with glycerol, a non-toxic, environmentally-friendly solvent. Response surface methodology based on Box-Behnken design was used to optimize the experimental conditions for ultrasound-assisted extraction of berberine and anti-radical components from root bark. The independent variables were temperature (X), glycerol concentration (X), and ultrasound power (X), while the responses were berberine concentration and DPPH radical scavenging activity of the extracts (RSA IC). The response values of the extracts prepared at optimum conditions were (response, X, X, X): berberine yield (145.5 μg/mL; 80 °C, 50%, 144 W) and RSA IC (58.88 μL/mL; 80 °C, 30%, 720 W). The observed values deviated from the predicted values by -3.45% and 6.42% for berberine and RSA IC, respectively, thus indicating the validity of the selected models. The prepared extracts demonstrated antioxidant, anti-melanogenic, and anti-inflammatory activity, as well excellent α-glucosidase and α-amylase inhibitory activity. The displayed biological properties and lack of glycerol toxicity makes the prepared extracts suitable for direct inclusion into antidiabetic and dermatologic food supplements and topical products.
该植物富含小檗碱，这是一种异喹啉生物碱，具有降血糖活性，常局部用于皮肤相关问题。本工作旨在开发一种使用水和甘油（一种无毒、环保的溶剂）混合物的“绿色”小檗碱提取方法。基于 Box-Behnken 设计的响应面法被用于优化超声辅助提取根皮中小檗碱和抗自由基成分的实验条件。独立变量为温度（X）、甘油浓度（X）和超声功率（X），而响应值为提取物中小檗碱浓度和 DPPH 自由基清除活性（RSA IC）。在最佳条件下制备的提取物的响应值为（响应、X、X、X）：小檗碱得率（145.5 μg/mL；80°C、50%、144 W）和 RSA IC（58.88 μL/mL；80°C、30%、720 W）。小檗碱和 RSA IC 的观察值与预测值的偏差分别为-3.45%和 6.42%，这表明所选模型的有效性。所制备的提取物表现出抗氧化、抗黑色素生成和抗炎活性，以及出色的α-葡萄糖苷酶和α-淀粉酶抑制活性。所显示的生物特性和缺乏甘油毒性使得所制备的提取物适合直接纳入抗糖尿病和皮肤科食品补充剂和局部产品。
