Franzo Giovanni, Settypalli Tirumala B K, Agusi Ebere Roseann, Meseko Clement, Minoungou Germaine, Ouoba Bruno Lalidia, Habibata Zerbo Lamouni, Wade Abel, de Barros José Luís, Tshilenge Curé Georges, Gelaye Esayas, Yami Martha, Gizaw Daniel, Chibssa Tesfaye Rufael, Anahory Iolanda Vieira, Mapaco Lourenço P, Achá Sara J, Ijomanta Jeremiah, Jambol Anvou Rachael, Adedeji Adeyinka Jeremy, Luka Pam Dachung, Shamaki David, Diop Mariame, Bakhoum Mame Thierno, Lo Modou Moustapha, Chang'a Jelly S, Magidanga Bishop, Mayenga Charles, Ziba Maureen Wakwamba, Dautu George, Masembe Charles, Achenbach Jenna, Molini Umberto, Cattoli Giovanni, Lamien Charles E, Dundon William G
Department of Animal Medicine, Production and Health, University of Padova, Legnaro, Italy.
Animal Production and Health Laboratory, Animal Production and Health Section, Joint FAO/IAEA Centre, Department of Nuclear Sciences and Applications, International Atomic Energy Agency, Vienna, Austria.
Transbound Emerg Dis. 2022 Jul;69(4):e1142-e1152. doi: 10.1111/tbed.14400. Epub 2021 Dec 3.
Porcine circovirus-2 (PCV-2) is associated with several disease syndromes in domestic pigs that have a significant impact on global pig production and health. Currently, little is known about the status of PCV-2 in Africa. In this study, a total of 408 archived DNA samples collected from pigs in Burkina Faso, Cameroon, Cape Verde, Ethiopia, the Democratic Republic of the Congo, Mozambique, Nigeria, Senegal, Tanzania and Zambia between 2000 and 2018 were screened by PCR for the presence of PCV-2. Positive amplicons of the gene encoding the viral capsid protein (ORF2) were sequenced to determine the genotypes circulating in each country. Four of the nine currently known genotypes of PCV-2 were identified (i.e. PCV-2a, PCV-2b, PCV-2d and PCV-2 g) with more than one genotype being identified in Burkina Faso, Ethiopia, Nigeria, Mozambique, Senegal and Zambia. Additionally, a phylogeographic analysis which included 38 additional ORF2 gene sequences of PCV-2s previously identified in Mozambique, Namibia and South Africa from 2014 to 2016 and 2019 to 2020 and available in public databases, demonstrated the existence of several African-specific clusters and estimated the approximate time of introduction of PCV-2s into Africa from other continents. This is the first in-depth study of PCV-2 in Africa and it has important implications for pig production at both the small-holder and commercial farm level on the continent.
猪圆环病毒2型（PCV-2）与家猪的多种疾病综合征有关，这些疾病对全球养猪生产和健康产生重大影响。目前，人们对非洲PCV-2的状况了解甚少。在本研究中，对2000年至2018年间从布基纳法索、喀麦隆、佛得角、埃塞俄比亚、刚果民主共和国、莫桑比克、尼日利亚、塞内加尔、坦桑尼亚和赞比亚的猪采集的408份存档DNA样本进行了PCR检测，以筛查PCV-2的存在情况。对编码病毒衣壳蛋白（ORF2）的基因的阳性扩增子进行测序，以确定每个国家流行的基因型。在PCV-2目前已知的9种基因型中，鉴定出了4种（即PCV-2a、PCV-2b、PCV-2d和PCV-2g），在布基纳法索、埃塞俄比亚、尼日利亚、莫桑比克、塞内加尔和赞比亚鉴定出了不止一种基因型。此外，系统发育地理学分析纳入了2014年至2016年以及2019年至2020年在莫桑比克、纳米比亚和南非先前鉴定的并可在公共数据库中获取的另外38条PCV-2的ORF2基因序列，结果表明存在几个非洲特有的聚类，并估计了PCV-2从其他大陆引入非洲的大致时间。这是对非洲PCV-2的首次深入研究，对该大陆的小农户和商业农场层面的养猪生产具有重要意义。
