基于自立液膜 SERS 基底的体液中 6-硫代鸟嘌呤的定量检测。
Quantitative detection of 6-thioguanine in body fluids based on a free-standing liquid membrane SERS substrate.
机构信息
Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, 611756, Sichuan, China.
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, 610031, Sichuan, China.
出版信息
Anal Bioanal Chem. 2022 Feb;414(4):1663-1670. doi: 10.1007/s00216-021-03790-x. Epub 2021 Nov 23.
The adverse reactions caused by 6-thioguanine (6-TG) in anti-cancer treatment are closely related to the dose, leading to the urgent need for clinical monitoring of its concentration. In this work, a highly reproducible free-standing liquid membrane (FLM) surface-enhanced Raman spectroscopy (SERS) substrate was developed to detect 6-TG in human urine and serum quantitatively. Briefly, a prepared sample was adjusted to pH 2 and mixed with concentrated core-shell bimetallic nanoparticle (AgAu NP) suspension. The Au/Ag ratio of the AgAu NPs was optimized. Then the mixture was formed into an FLM using a custom mold. The relative standard deviation (RSD) of the experimental results can be stabilized below 10% (n ≥ 10). The R of the calibration curve in the range of 10 ~ 100 μg kg was 0.988. In addition, the limit of detection (LOD) (3σ/k) of 6-TG was 5 μg kg. The FLM SERS platform has been successfully applied to the rapid and reliable analysis of 6-TG spiked in body fluids.
在抗癌治疗中，6-硫代鸟嘌呤（6-TG）引起的不良反应与剂量密切相关，这就迫切需要对其浓度进行临床监测。在这项工作中，开发了一种重现性好的自由站立液膜（FLM）表面增强拉曼光谱（SERS）基底，用于定量检测人尿和血清中的 6-TG。简要地，将制备的样品调节至 pH 2，并与浓缩的核壳双金属纳米粒子（AgAu NP）悬浮液混合。优化了 AgAu NPs 的 Au/Ag 比。然后，使用定制模具将混合物制成 FLM。实验结果的相对标准偏差（RSD）可以稳定在 10%以下（n≥10）。在 10~100μgkg 的范围内，校准曲线的 R 值为 0.988。此外，6-TG 的检测限（LOD）（3σ/k）为 5μgkg。FLM SERS 平台已成功应用于快速可靠地分析体液中添加的 6-TG。
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