Faculty of Geosciences and Environmental Engineering, Southwest Jiaotong University, Chengdu, 611756, Sichuan, China.
Department of Gastroenterology, West China Hospital, Sichuan University, Chengdu, 610031, Sichuan, China.
Anal Bioanal Chem. 2022 Feb;414(4):1663-1670. doi: 10.1007/s00216-021-03790-x. Epub 2021 Nov 23.
The adverse reactions caused by 6-thioguanine (6-TG) in anti-cancer treatment are closely related to the dose, leading to the urgent need for clinical monitoring of its concentration. In this work, a highly reproducible free-standing liquid membrane (FLM) surface-enhanced Raman spectroscopy (SERS) substrate was developed to detect 6-TG in human urine and serum quantitatively. Briefly, a prepared sample was adjusted to pH 2 and mixed with concentrated core-shell bimetallic nanoparticle (AgAu NP) suspension. The Au/Ag ratio of the AgAu NPs was optimized. Then the mixture was formed into an FLM using a custom mold. The relative standard deviation (RSD) of the experimental results can be stabilized below 10% (n ≥ 10). The R of the calibration curve in the range of 10 ~ 100 μg kg was 0.988. In addition, the limit of detection (LOD) (3σ/k) of 6-TG was 5 μg kg. The FLM SERS platform has been successfully applied to the rapid and reliable analysis of 6-TG spiked in body fluids.
在抗癌治疗中，6-硫代鸟嘌呤（6-TG）引起的不良反应与剂量密切相关，这就迫切需要对其浓度进行临床监测。在这项工作中，开发了一种重现性好的自由站立液膜（FLM）表面增强拉曼光谱（SERS）基底，用于定量检测人尿和血清中的 6-TG。简要地，将制备的样品调节至 pH 2，并与浓缩的核壳双金属纳米粒子（AgAu NP）悬浮液混合。优化了 AgAu NPs 的 Au/Ag 比。然后，使用定制模具将混合物制成 FLM。实验结果的相对标准偏差（RSD）可以稳定在 10%以下（n≥10）。在 10~100μgkg 的范围内，校准曲线的 R 值为 0.988。此外，6-TG 的检测限（LOD）（3σ/k）为 5μgkg。FLM SERS 平台已成功应用于快速可靠地分析体液中添加的 6-TG。
