College of Plant Protection, Northwest A&F University, Yangling, Shaanxi 712100, China.
Engineering and Technology Centers of Biopesticide in Shaanxi, Yangling, Shaanxi 712100, China.
J Agric Food Chem. 2021 Dec 8;69(48):14520-14529. doi: 10.1021/acs.jafc.1c04385. Epub 2021 Nov 23.
extracts showed strong antibacterial activity against selected phytopathogenic bacteria. Bioguided isolation was conducted to obtain 11 phenanthrene derivatives (-), 4 phenylpropanoids (-), a flavonoid (), and a steroid (), including a new phenanthrene derivative (). bioassay results showed that phenanthrene derivatives are the main active components of extracts. The new compound aristoloxazine C () was found to exhibit outstanding antibacterial activity against , , , , and , with MIC values of 0.05, 2.5, 2.5, 5, and 6.25 μg/mL, respectively. These values were significantly higher than that of the positive control, streptomycin sulfate. Aristoloxazine C () also demonstrated an excellent control effect on tobacco bacterial wilt. Physiological and biochemical experiments combined with electron microscopy showed that the antibacterial activity of aristoloxazine C () was primarily related to the destruction of the bacterial cell wall structure. Thus, aristoloxazine C () may have the potential to be used as a template for the development of new bactericides or as a probe for the discovery of new antimicrobial targets.
提取物对选定的植物病原菌表现出强烈的抗菌活性。进行了生物导向分离以获得 11 种菲衍生物（-）、4 种苯丙素（-）、一种黄酮（）和一种甾体（），包括一种新的菲衍生物（）。生物测定结果表明，菲衍生物是提取物的主要活性成分。新化合物 aristoloxazine C（）被发现对 、 、 、 、 和 具有出色的抗菌活性，MIC 值分别为 0.05、2.5、2.5、5 和 6.25 μg/mL，明显高于阳性对照硫酸链霉素。Aristoloxazine C（）对烟草细菌性萎蔫病也表现出极好的防治效果。生理生化实验结合电子显微镜观察表明，aristoloxazine C（）的抗菌活性主要与破坏细菌细胞壁结构有关。因此，aristoloxazine C（）可能具有作为开发新型杀菌剂的模板或发现新抗菌靶标的潜力。
