[Analysis on epidemiological characteristics of multidrug-resistant Kentucky in Beijing, 2010-2020].

To investigate the epidemiological, drug resistance and molecular characteristics of (.) Kentucky strains isolated from diarrheal patients in Beijing. The drug susceptibility of 22 Kentucky strains isolated in Beijing during 2010-2020 was tested by using the micro broth dilution method. The multilocus sequence typing (MLST), drug resistance genes and genomic island (SGI) identifications of the strains were performed by whole-genome sequencing (WGS).The pulsed field gel electrophoresis (PFGE) was performed to analyze the molecular epidemiological characteristics of the isolates. The 22 strains were highly resistant to 8-22 kinds of antibiotics, especially to ciprofloxacin, cephalosporins and azithromycin, ., showing a super high level of multidrug resistance and 21 strains were positive for extended-spetrum beta-lactamase (ESBL). The WGS analysis revealed that all the isolates belonged to ST198, carrying SGI1-K. The drug resistance genes , and were identified in all strains and Quinolone resistance-determining regions (QRDRs) showed 2 mutations in (S83F, D87 N) and 3 mutations in the gene (T57S, S80I, T255S). The resistance genes associated with -lactam antibiotics (, , , , , , ), resistance genes associated with aminoglycosides [, , , , , -, -, -,] as well as , , and had significant differences in the strains of different years, which were highly consistent with the drug-resistant phenotype. Furthermore, phylogenetic analysis showed that the similarity of the 22 strains was more than 85%, and the strains were highly homologous to CIP-resistant ST198-X1 circulating in the world. In the process of spread, the drug resistance and PFGE spectrums had changed, two clusters had formed. The Kentucky strains isolated in Beijing shared high homology with the multidrug-resistant strain ST198-X1-SGI-1K isolated in the world, which has maintained a low level of spread since 2016, causing sporadic infections and clusters of diarrhea, and has serious resistances to fluoroquinolones, ESBLs and azithromycin. The surveillance for multidrug-resistant Kentucky should be strengthened.