Jia J, Yuan Q, Hui J W, Liang J W, Wang X, Liu H H, Wang Z G, Dai X Q, Hao B, Gao R Q, Jiang F C, Ma H L
Qingdao Municipal Center for Disease Control and Prevention, Qingdao 266033,China.
Shandong Center for Disease Control and Prevention, Ji'nan 250014,China.
Zhonghua Liu Xing Bing Xue Za Zhi. 2021 Aug 10;42(8):1360-1364. doi: 10.3760/cma.j.cn112338-20210209-00107.
To investigate the contamination status of SARS-CoV-2 in imported frozen seafood from a Russia cargo ship in Qingdao and to analyze the risk factors for infection in local stevedores. The method of "two-stage, full coverage and mixed sampling" was used to collect the seafood packaging samples for the nucleic acid detection of SARS-CoV-2 by real-time fluorescent quantitative RT-PCR. A unified questionnaire was designed to investigate 71 stevedores in two shifts through telephone interview. The stevedores were divided into two groups, with 23 in the shit with two infections was group A and 48 in the shift without infection was group B. Software Epi Info7.2 was used to identify the risk factors for SARS-CoV-2 infections in the stevedores. In the frozen seafood from a Russia cargo ship, the total positive rate of SARS-CoV-2 nucleic acid in the frozen seafood was 11.53% (106/919). The positive rate of SARS-CoV-2 nucleic acid in the frozen seafood unloaded by group A (14.29%,70/490) was significantly higher than that in the frozen seafood unloaded by group B (8.39%,36/429)(=7.79,=0.01) and the viral loads detected in the frozen seafood unloaded by group A were higher than those detected in the frozen seafood unloaded by group B. The scores of personal protection and behaviors in the stevedores in group A were significantly lower than those in group B (<0.05), and toilet use, smoking and improper hand washing before meals were the risk factors for the infection. The imported frozen seafood was contaminated by SARS-CoV-2 and the contamination distribution was uneven. Supervision and management of personal occupational protection and behaviors of workers engaged in imported frozen food transportation should be strengthened. It is suggested that a closed-loop monitoring and management system for the whole process of "fishing-transport- loading/unloading" should be established by marine fishery authority.
为调查青岛一艘俄罗斯货船进口冷冻海鲜中新型冠状病毒（SARS-CoV-2）污染状况，并分析当地装卸工人感染的危险因素。采用“两阶段、全覆盖、混合采样”方法采集海鲜包装样本，通过实时荧光定量RT-PCR进行SARS-CoV-2核酸检测。设计统一问卷，通过电话访谈对两班71名装卸工人进行调查。将装卸工人分为两组，A组为有2例感染的班次中的23人，B组为无感染班次中的48人。使用Epi Info7.2软件识别装卸工人感染SARS-CoV-2的危险因素。在一艘俄罗斯货船的冷冻海鲜中，冷冻海鲜SARS-CoV-2核酸总阳性率为11.53%（106/919）。A组卸载的冷冻海鲜中SARS-CoV-2核酸阳性率（14.29%，70/490）显著高于B组卸载的冷冻海鲜（8.39%，36/429）（χ² = 7.79，P = 0.01），且A组卸载的冷冻海鲜中检测到的病毒载量高于B组。A组装卸工人个人防护和行为得分显著低于B组（P<0.05），使用厕所、吸烟和饭前洗手不当是感染的危险因素。进口冷冻海鲜受到SARS-CoV-2污染，且污染分布不均。应加强对从事进口冷冻食品运输工人个人职业防护和行为的监督管理。建议海洋渔业部门建立“捕捞-运输-装卸”全过程闭环监测管理系统。
