Kleinrok Z, Jagiełlłlo-Wójtowicz E, Sieklucka-Dziuba M
Arch Immunol Ther Exp (Warsz). 1977;25(6):829-37.
It was found that fluorostigmine (DFP) in doses 0-4 mg/kg i.p. reduced significantly tryptophan pyrolase activity in mouse liver homogenates.se liver homogenates 120 and 180 min after the administration of the drug. On the other hand, fluorostigmine administered in doses 0-8 mg/kg i.p. increases the activity of the enzyme studied at 30 and 60 min after the injection. DFP (0.4 mg/kg i.p.) given in combination with atropine (10 mg/kg i.p.) causes a significant increase in enzyme activity determined 30, 60, 120 and 180 min after the administration of the drug. Toxogonine (in doses 5 mg/kg s.c.) given together with DFP (0.4 mg/kg i.p.) intensifies the activity of tryplophan pyrolase at 180 min after their administration. Simultaneous treatment with toxogonine (5 mg/kg s.c.), atropine (10 mg/kg i.p.) and DFP (0.4 MG/KG I.P.) reduced significantly the activity of the enzyme studied at all time of determination. Moreover, the experiment in vitro showed that DFP in concentrations of 4x10(-9) M; 8X10(-9) M; 1.6X10(-8) M and 3.2x10(-8) M increased tryptophan pyrolase activity in mouse liver homogenates.
结果发现，腹腔注射剂量为0 - 4mg/kg的氟磷定（DFP）能显著降低给药后120分钟和180分钟时小鼠肝脏匀浆中色氨酸吡咯酶的活性。另一方面，腹腔注射剂量为0 - 8mg/kg的氟磷定能使注射后30分钟和60分钟时所研究酶的活性增加。腹腔注射0.4mg/kg的DFP与腹腔注射10mg/kg的阿托品联合使用，会使给药后30分钟、60分钟、120分钟和180分钟时所测定的酶活性显著增加。皮下注射5mg/kg的解磷定与腹腔注射0.4mg/kg的DFP一起使用，会在给药后180分钟时增强色氨酸吡咯酶的活性。同时用皮下注射5mg/kg的解磷定、腹腔注射10mg/kg的阿托品和腹腔注射0.4mg/kg的DFP进行治疗，会在所有测定时间显著降低所研究酶的活性。此外，体外实验表明，浓度为4×10⁻⁹M、8×10⁻⁹M、1.6×10⁻⁸M和3.2×10⁻⁸M的DFP能增加小鼠肝脏匀浆中色氨酸吡咯酶的活性。
