Xiao Huiwen, Fan Yao, Li Yuan, Dong Jiali, Zhang Shuqin, Wang Bin, Liu Jia, Liu Xingzhong, Fan Saijun, Guan Jian, Cui Ming
Department of Microbiology, College of Life Sciences, Nankai University, 94 Weijin Road, Tianjin 300071, China.
Tianjin Key Laboratory of Radiation Medicine and Molecular Nuclear Medicine, Institute of Radiation Medicine, Chinese Academy of Medical Sciences and Peking Union Medical College, 238 Baidi Road, Tianjin 300192, China.
Comput Struct Biotechnol J. 2021 Oct 25;19:5898-5910. doi: 10.1016/j.csbj.2021.10.028. eCollection 2021.
Oral mucositis is a common radiotherapy-induced complication among nasal, oral and laryngeal cancer (NOALC) patients. This complication leads to decreased quality of life and has few treatments. Here, fractionated radiation was performed to mimic radiotherapy for NOALCs in mouse models. Oral microbiota transplantation (OMT) mitigated oral mucositis, as judged by reconstructed epithelium and tongue papillae, fewer infiltrated leukocytes and more proliferative cells in the oral epithelium. The gut microbiota impacted oral mucositis progression, and OMT restructured oral and gut bacteria configurations and reprogrammed the gene expression profile of tongue tissues. silencing of glossal S100 calcium binding protein A9 debilitated the radioprotection of OMT. In light of clinical samples, we identified that patients with different alteration trends of frequency presented different primary lesions and prognoses of NOALC following radiotherapy. Together, our findings provide new insights into the oral-gut microbiota axis and underpin the suggestion that OMT might be harnessed as a novel remedy to fight against oral mucositis in NOALC patients following radiotherapy in preclinical settings. Of note, oral microorganisms, such as , might be employed as biomarkers to predict the prognosis of NOALC with radiotherapy.
口腔黏膜炎是鼻、口、喉癌（NOALC）患者常见的放疗并发症。这种并发症会导致生活质量下降，且治疗方法有限。在此，在小鼠模型中进行分次放疗以模拟NOALC的放疗过程。通过重建的上皮组织和舌乳头、较少浸润的白细胞以及口腔上皮中较多的增殖细胞判断，口腔微生物群移植（OMT）减轻了口腔黏膜炎。肠道微生物群影响口腔黏膜炎的进展，OMT重塑了口腔和肠道细菌结构，并重新编程了舌组织的基因表达谱。舌部S100钙结合蛋白A9的沉默削弱了OMT的放射保护作用。根据临床样本，我们发现频率变化趋势不同的患者在放疗后出现不同的NOALC原发灶和预后。总之，我们的研究结果为口腔-肠道微生物群轴提供了新的见解，并支持了在临床前环境中，OMT可能被用作一种新的疗法来对抗放疗后NOALC患者的口腔黏膜炎这一建议。值得注意的是，诸如 等口腔微生物可能被用作预测放疗后NOALC预后的生物标志物。
