Division of Surgery, Department of Clinical Science, Intervention and Technology, Karolinska Institutet, Stockholm, Sweden.
Department of Surgical and Perioperative Sciences, Surgery, Umeå University, Umeå, Sweden.
Gut Microbes. 2021 Jan-Dec;13(1):1983101. doi: 10.1080/19490976.2021.1983101.
Emerging research suggests gut microbiome may play a role in pancreatic cancer initiation and progression, but cultivation of the cancer microbiome remains challenging. This pilot study aims to investigate the possibility to cultivate pancreatic microbiome from pancreatic cystic lesions associated with invasive cancer. Intra-operatively acquired pancreatic cyst fluid samples showed culture-positivity mainly in the intraductal papillary mucinous neoplasm (IPMN) group of lesions. MALDI-TOF MS profiling analysis shows Gammaproteobacteria and Bacilli dominate among individual bacteria isolates. Among cultivated bacteria, Gammaproteobacteria, particularly , but also and , demonstrate consistent pathogenic properties in pancreatic cell lines tested in co-culture models. Pathogenic properties include intracellular survival capability, cell death induction, or causing DNA double-strand breaks in the surviving cells resembling genotoxic effects. This study provides new insights into the role of the pancreatic microbiota in the intriguing link between pancreatic cystic lesions and cancer.
新兴研究表明，肠道微生物组可能在胰腺癌的发生和发展中起作用，但培养癌症微生物组仍然具有挑战性。本研究旨在探讨从伴有浸润性癌的胰腺囊性病变中培养胰腺微生物组的可能性。术中获得的胰腺囊液样本显示，培养阳性主要见于导管内乳头状黏液性肿瘤（IPMN）病变组。基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）分析显示，γ-变形菌门和芽孢杆菌门在单个细菌分离株中占主导地位。在共培养模型中，在被测试的胰腺细胞系中，培养的细菌中γ-变形菌门，特别是 、 和 ，表现出一致的致病性。致病性包括细胞内生存能力、诱导细胞死亡或导致存活细胞中的 DNA 双链断裂，类似于遗传毒性作用。本研究为胰腺囊性病变与癌症之间的这种有趣联系中胰腺微生物组的作用提供了新的见解。
