Department of Otorhinolaryngology and Head and Neck Surgery, Chengdu Ren Pin Otorhinolaryngology Hospital, Chengdu, China.
Cell Mol Biol (Noisy-le-grand). 2021 Aug 31;67(2):114-120. doi: 10.14715/cmb/2021.67.2.17.
To investigate the relationship between miR-410-3p, miR-34c and nasopharyngeal carcinoma development, we detected the expression of miR-410-3p and miR-34c in nasopharyngeal carcinoma tissues and evaluate its clinical value as a molecular marker for predicting the prognosis in patients with nasopharyngeal carcinoma through clinical case study. To identify the role and mechanism of miR-410-3p and miR-34c in nasopharyngeal carcinoma development and progression. The expression of miR-410-3p and miR-34cin 300 cases of nasopharyngeal carcinoma tissues and 30 cases of paired adjacent normal breast tissues was detected by RT-qPCR. The paired t-test was used to compare the differences of miR-410-3pand miR-34c levels between the nasopharyngeal carcinoma and normal groups. The Chi-square test was used to compare the differences between miR-410-3p and miR-34c expression and clinicopathological factors. The Kaplan-Meier survival curve was used to analyze the relationship between miR-410-3p and miR-34c expression and 5-year overall survival (OS). The Cox proportional hazards regression model was used to evaluate the prognostic value. The results were validated by TCGA database. The expression of miR-410-3p was down-regulated in nasopharyngeal carcinoma tissues compared with that of paired normal tissues (P<0.001). The patients with lower miR-410-3p expression have a higher ratio of positive lymph node status (P=0.039) and a poorer 5-year disease-free survival (P=0.001) and 5-year overall survival (P = 0.002). The expression of mi R-34c was down-regulated in nasopharyngeal carcinoma tissues compared with that of paired normal tissues (P<0.001). Up-regulation of the miR-34c inhibited the viability of paired normal tissues (P<0. 01), but there was no significant change in migration of the paired normal tissues. Downregulation of mi R-34c promoted the viability and migration of paired normal tissues (P<0. 05). The expression of miR-410-3p and miR-34c levels are predictors for the OS in patients with nasopharyngeal carcinoma. The expression of miR-410-3p and miR-34c are molecular markers of early nasopharyngeal carcinoma metastasis and an independent prognostic biomarker for patients with nasopharyngeal carcinoma.
为了研究 miR-410-3p、miR-34c 与鼻咽癌发展之间的关系，我们通过临床病例研究检测了鼻咽癌组织中 miR-410-3p 和 miR-34c 的表达，并评估其作为预测鼻咽癌患者预后的分子标志物的临床价值。为了确定 miR-410-3p 和 miR-34c 在鼻咽癌发展和进展中的作用和机制。通过 RT-qPCR 检测了 300 例鼻咽癌组织和 30 例配对的相邻正常乳腺组织中 miR-410-3p 和 miR-34c 的表达。采用配对 t 检验比较鼻咽癌组和正常组 miR-410-3p 和 miR-34c 水平的差异。采用卡方检验比较 miR-410-3p 和 miR-34c 表达与临床病理因素的差异。采用 Kaplan-Meier 生存曲线分析 miR-410-3p 和 miR-34c 表达与 5 年总生存率（OS）的关系。采用 Cox 比例风险回归模型评估预后价值。通过 TCGA 数据库进行验证。miR-410-3p 在鼻咽癌组织中的表达低于配对正常组织（P<0.001）。miR-410-3p 低表达的患者阳性淋巴结状态的比例更高（P=0.039），5 年无病生存率（P=0.001）和 5 年总生存率（P=0.002）更差。miR-34c 在鼻咽癌组织中的表达低于配对正常组织（P<0.001）。miR-34c 的上调抑制了配对正常组织的活力（P<0.01），但对配对正常组织的迁移没有明显影响。miR-34c 的下调促进了配对正常组织的活力和迁移（P<0.05）。miR-410-3p 和 miR-34c 的表达水平是鼻咽癌患者 OS 的预测因子。miR-410-3p 和 miR-34c 的表达是早期鼻咽癌转移的分子标志物，也是鼻咽癌患者独立的预后生物标志物。
