Department of Chemistry, Brandeis University, 415 South St., Waltham, MA 02454, USA.
Chem Commun (Camb). 2021 Dec 3;57(96):12870-12879. doi: 10.1039/d1cc05565h.
Enzymatic noncovalent synthesis enables the spatiotemporal control of multimolecular crowding in cells, thus offering a unique opportunity for modulating cellular functions. This article introduces some representative enzymes and molecular building blocks for generating peptide assemblies as multimolecular crowding in cells, highlights the relevant biomedical applications, such as anticancer therapy, molecular imaging, trafficking proteins, genetic engineering, artificial intracellular filaments, cell morphogenesis, and antibacterial, and briefly discusses the promises of ENS as a multistep molecular process in biology and medicine.
酶促非共价合成能够实现细胞中多分子拥挤的时空控制，从而为调节细胞功能提供了独特的机会。本文介绍了一些代表性的酶和分子构建块，用于在细胞中生成肽组装体作为多分子拥挤，强调了相关的生物医学应用，如抗癌治疗、分子成像、转运蛋白、基因工程、人工细胞内丝、细胞形态发生和抗菌，并简要讨论了 ENS 作为生物学和医学中的多步分子过程的前景。
