采用柱后酶反应和离线荧光测定的高效液相色谱法分析人血清中的结合胆汁酸。
Analysis of conjugated bile acids in human serum by high-performance liquid chromatography using post-column enzyme reaction and off-line fluorimetric determination.
作者信息
Swobodnik W, Zhang Y Y, Klueppelberg U, Janowitz P, Wechsler J G, Fuerst P, Ditschuneit H
机构信息
University Clinic of Ulm, Department of Internal Medicine II, F.R.G.
出版信息
J Chromatogr. 1987 Dec 25;423:75-84. doi: 10.1016/0378-4347(87)80329-8.
An assay for glycine and taurine conjugates of cholic (GC, TC), chenodeoxycholic (GCDC, TCDC), deoxycholic (GDC, TDC) and ursodeoxycholic (GUDC, TUDC) acids in human serum by high-performance liquid chromatography using an immobilized 3-alpha-hydroxysteroid dehydrogenase post-column reaction and an off-line spectrofluorimetric detection is described. The detection limit is 0.2 microM. Recoveries of extraction and chromatographic analysis range from 89.50 to 91.68% and from 96.75 to 98.83%, respectively. The analysis in serum reveals a coefficient of variation for reproducibility between 16.2% for GC and 4.7% for TDC; inter-assay variability ranges between 4.06% for TCDC and 8.24% for TUDC.
本文描述了一种利用固定化3-α-羟基类固醇脱氢酶柱后反应和离线荧光光谱检测的高效液相色谱法，用于测定人血清中胆酸（GC、TC）、鹅去氧胆酸（GCDC、TCDC）、脱氧胆酸（GDC、TDC）和熊去氧胆酸（GUDC、TUDC）的甘氨酸和牛磺酸共轭物。检测限为0.2微摩尔。萃取和色谱分析的回收率分别为89.50%至91.68%和96.75%至98.83%。血清分析显示，GC的重现性变异系数为16.2%，TDC为4.7%；批间变异在TCDC的4.06%至TUDC的8.24%之间。
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