Swobodnik W, Zhang Y Y, Klueppelberg U, Janowitz P, Wechsler J G, Fuerst P, Ditschuneit H
University Clinic of Ulm, Department of Internal Medicine II, F.R.G.
J Chromatogr. 1987 Dec 25;423:75-84. doi: 10.1016/0378-4347(87)80329-8.
An assay for glycine and taurine conjugates of cholic (GC, TC), chenodeoxycholic (GCDC, TCDC), deoxycholic (GDC, TDC) and ursodeoxycholic (GUDC, TUDC) acids in human serum by high-performance liquid chromatography using an immobilized 3-alpha-hydroxysteroid dehydrogenase post-column reaction and an off-line spectrofluorimetric detection is described. The detection limit is 0.2 microM. Recoveries of extraction and chromatographic analysis range from 89.50 to 91.68% and from 96.75 to 98.83%, respectively. The analysis in serum reveals a coefficient of variation for reproducibility between 16.2% for GC and 4.7% for TDC; inter-assay variability ranges between 4.06% for TCDC and 8.24% for TUDC.
本文描述了一种利用固定化3-α-羟基类固醇脱氢酶柱后反应和离线荧光光谱检测的高效液相色谱法，用于测定人血清中胆酸（GC、TC）、鹅去氧胆酸（GCDC、TCDC）、脱氧胆酸（GDC、TDC）和熊去氧胆酸（GUDC、TUDC）的甘氨酸和牛磺酸共轭物。检测限为0.2微摩尔。萃取和色谱分析的回收率分别为89.50%至91.68%和96.75%至98.83%。血清分析显示，GC的重现性变异系数为16.2%，TDC为4.7%；批间变异在TCDC的4.06%至TUDC的8.24%之间。
