Section of Paediatric Infectious Diseases, Department of Infectious Diseases, Imperial College London, London, UK.
Department of Production Animal Clinical Sciences Faculty of Veterinary Medicine, Norwegian University of Life Sciences, Ås, Norway.
Microb Genom. 2021 Nov;7(11). doi: 10.1099/mgen.0.000687.
We report here the complete genome sequence of the widely studied serovar 8 reference strain 405, generated using the Pacific Biosciences (PacBio) RS II platform. Furthermore, we compared draft sequences generated by Illumina sequencing of six stocks of this strain, including the same original stock used to generate the PacBio sequence, held in different countries and found little genetic variation, with only three SNPs identified, all within the gene. However, sequences of two small plasmids, pARD3079 and p405tetH, detected by Illumina sequencing of the draft genomes were not identified in the PacBio sequence of the reference strain.
我们在此报告广泛研究的血清型 8 参考菌株 405 的完整基因组序列，该序列是使用太平洋生物科学公司（PacBio）RS II 平台生成的。此外，我们比较了使用 Illumina 测序生成的六株该菌株的草图序列，包括用于生成 PacBio 序列的相同原始菌株，这些菌株保存在不同的国家，发现遗传变异很小，仅鉴定出三个 SNP，全部位于基因内。然而，Illumina 测序的两个小质粒 pARD3079 和 p405tetH 的序列在参考菌株的 PacBio 序列中未被鉴定。
