Chen Shi, Hermann Thomas
J Am Chem Soc. 2021 Dec 8;143(48):20356-20362. doi: 10.1021/jacs.1c09739. Epub 2021 Nov 25.
The preparation of nucleic acid nanostructures has relied predominantly on procedures of additive fabrication in which complex architectures are assembled by concerted self-assembly and sequential addition of building blocks. We had previously established RNA-DNA hybrid nanoshapes with modular architectures that enable multistep synthetic approaches inspired by organic molecular synthesis where additive and transformative steps are used to prepare complex molecular architectures. We report the establishment of module replacement and strand exchange as synthetic transformations in nucleic acid hybrid nanoshapes, which are enabled by minimally destabilizing sequence elements such as a single unpaired overhang nucleotide or a mismatch base pair. Module exchange facilitated by thermodynamic lability triggers adds a powerful transformative approach to the repertoire of additive and transformative synthetic methods for the preparation of complex composite materials.
核酸纳米结构的制备主要依赖于加成制造程序，其中复杂的结构通过协同自组装和顺序添加构建块来组装。我们之前已经建立了具有模块化结构的RNA-DNA杂交纳米形状，这种结构能够实现受有机分子合成启发的多步合成方法，其中加成和转化步骤用于制备复杂的分子结构。我们报告了在核酸杂交纳米形状中建立模块替换和链交换作为合成转化方法，这是由最小程度破坏稳定性的序列元件（如单个未配对的突出核苷酸或错配碱基对）实现的。由热力学不稳定性触发的模块交换为制备复杂复合材料的加成和转化合成方法库增添了一种强大的转化方法。
