中国襄阳手足口病流行中新型 CV-A5 重组的出现。
Emergence of a novel recombinant of CV-A5 in HFMD epidemics in Xiangyang, China.
机构信息
Wuhan Institute of Biological Products, Co. Ltd, Wuhan, 430207, People's Republic of China.
Xiangyang Center for Disease Control and Prevention, Hubei, People's Republic of China.
出版信息
BMC Med Genomics. 2021 Nov 24;14(1):279. doi: 10.1186/s12920-021-01107-6.
BACKGROUND
Hand, foot and mouth disease (HFMD) is caused by a variety of enterovirus serotypes and the etiological spectrum worldwide has changed since a large scale of outbreaks occurred in 1997.
METHODS
A large number of clinical specimens of HFMD patients were collected in Xiangyang and genotyping was performed by qRT-PCR, conventional PCR amplification and sequencing. Among the 146 CV-A5 detected cases, the complete genome sequences of representative strains were determined for genotyping and for recombination analysis.
RESULTS
It was found that CV-A5 was one of the six major serotypes that caused the epidemic from October 2016 to December 2017. Phylogenetic analyses based on the VP1 sequences showed that these CV-A5 belonged to the genotype D which dominantly circulated in China. Recombination occurred between the CV-A5 and CV-A2 strains with a breakpoint in the 2A region at the nucleotide 3791.
CONCLUSIONS
The result may explain the emergence of CV-A5 as one of the major pathogens of HFMD. A multivalent vaccine against HFMD is urgently needed to control the disease and to prevent emerging and spreading of new recombinants.
背景
手足口病（HFMD）是由多种肠道病毒血清型引起的，自 1997 年大规模爆发以来，全球的病因谱发生了变化。
方法
在襄阳收集了大量手足口病患者的临床标本，通过 qRT-PCR、常规 PCR 扩增和测序进行基因分型。在检测到的 146 例 CV-A5 中，确定了代表性菌株的完整基因组序列进行基因分型和重组分析。
结果
发现 CV-A5 是 2016 年 10 月至 2017 年 12 月引起流行的六大血清型之一。基于 VP1 序列的系统进化分析表明，这些 CV-A5 属于在中国流行的 D 基因型。CV-A5 和 CV-A2 株之间发生了重组，断点位于核苷酸 3791 的 2A 区。
结论
这一结果可能解释了 CV-A5 作为手足口病主要病原体之一的出现。迫切需要开发针对手足口病的多价疫苗，以控制该疾病，并防止新重组体的出现和传播。
Zotero 插件