检测和遗传特征分析 2014-2016 年日本儿童急性肠胃炎相关肠病毒株
Detection and genetic characterization of enterovirus strains circulating among children with acute gastroenteritis in Japan during 2014-2016.
机构信息
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
Division of Microbiology, Department of Pathology and Microbiology, Nihon University School of Medicine, Tokyo, Japan.
出版信息
Infect Genet Evol. 2018 Jul;61:16-19. doi: 10.1016/j.meegid.2018.03.009. Epub 2018 Mar 11.
Of 972 stool samples collected from infants and children with acute gastroenteritis in six different regions (Hokkaido, Tokyo, Shizuoka, Kyoto, Osaka, and Saga) of Japan during 2-year period from July 2014 to June 2016, 63 samples (6.5%) determined to be positive for enterovirus by multiplex RT-PCR were subjected to genotype determination based on the partial VP1 region using the CODEHOP method. Sixty-two strains were succeeded in genotyping and seventeen EV types were identified. The majority of the studied strains belonged to EV-A (30 of 62; 48.4%) and EV-B (31 of 62, 50%) species, and the remaining strain was of the EV-D species. The most frequently detected type was Coxsackievirus A5 (CV-A5) in 2014-2015 while was CV-B5 in 2015-2016. This study provides an insight into the genetic diversity of EV with the predominance of EV-A and EV-B species in Japanese infants and children with acute gastroenteritis during 2014-2016.
在 2014 年 7 月至 2016 年 6 月的 2 年期间，从日本六个不同地区（北海道、东京、静冈、京都、大阪和佐贺）的急性肠胃炎婴儿和儿童中收集了 972 份粪便样本，通过多重 RT-PCR 检测到 63 份（6.5%）为肠道病毒阳性样本，基于部分 VP1 区域，采用 CODEHOP 方法对其进行基因分型。成功对 62 株进行了基因分型，鉴定出 17 种 EV 型。研究的大多数菌株属于 EV-A（62 株中的 30 株；48.4%）和 EV-B（62 株中的 31 株，50%）种，其余一株属于 EV-D 种。在 2014-2015 年期间，最常检测到的类型是柯萨奇病毒 A5（CV-A5），而在 2015-2016 年期间，最常检测到的是 CV-B5。本研究深入了解了 2014-2016 年日本急性肠胃炎婴儿和儿童中肠道病毒的遗传多样性，以 EV-A 和 EV-B 为主。
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