来自酿酒酵母（面包酵母）的脂质二磷酸N-乙酰葡糖胺中存在多萜醇。
The presence of dolichol in a lipid diphosphate N-acetylglucosamine from Saccharomyces cerevisiae (baker's yeast).
作者信息
Reuvers F, Boer P, Hemming F W
出版信息
Biochem J. 1978 Mar 1;169(3):505-8. doi: 10.1042/bj1690505.
Abstract
The lipid moiety of a lipid diphosphate N-acetylglucosamine, an intermediate in glycosylation of proteins, was studied. Ozonolysis of the compound gave evidence for an alpha-saturated isoprene unit. Alkaline hydrolysis of the glycolipid, followed by high-pressure liquid chromatography, showed the presence of a series of polyprenol homologues identical with those isolated directly from Saccharomyces cerevisiae (baker's yeast). No particular homologue was preferred in the enzymic transfer of N-acetylglucosamine 1-phosphate to endogenous dolichol monophosphate.
摘要
对蛋白质糖基化过程中的中间体——脂二磷酸N-乙酰葡糖胺的脂质部分进行了研究。该化合物的臭氧化反应证明存在一个α-饱和异戊二烯单元。糖脂经碱性水解后，通过高压液相色谱分析，结果表明存在一系列与直接从酿酒酵母（面包酵母）中分离出的聚戊烯醇同系物相同的物质。在N-乙酰葡糖胺1-磷酸向内源性磷酸多萜醇的酶促转移过程中，未发现对特定同系物有偏好。
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3
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