采用高压液相色谱法分析人体组织中的多萜醇。

Analysis of dolichol in human tissues by high pressure liquid chromatography.

作者信息

Freeman D J, Rupar C A, Carroll K K

出版信息

Lipids. 1980 Mar;15(3):191-3. doi: 10.1007/BF02540968.

Abstract

Dolichol from human liver was shown by reverse-phase high pressure liquid chromatography to consist of a series of homologues ranging in length from 17 to 23 isoprene units. The two major components, corresponding to 19 and 20 isoprene units, respectively, were isolated and identified by mass spectrometry. Dolichyl palmitate, synthesized from liver dolichol, showed an identical series of peaks with longer retention times. Attempts to chromatography dolichyl phosphate under similar conditions were unsuccessful. Dolichol from uterine tissue and several other human tissues showed a shift toward shorter chain length, with a predominance of homologues containing 18 and 19 isoprene units.

摘要

通过反相高压液相色谱法显示，人肝脏中的多萜醇由一系列长度从17至23个异戊二烯单元的同系物组成。通过质谱法分离并鉴定了分别对应于19和20个异戊二烯单元的两种主要成分。由肝脏多萜醇合成的棕榈酸多萜酯显示出一系列相同的峰，但保留时间更长。在类似条件下对磷酸多萜醇进行色谱分析的尝试未成功。来自子宫组织和其他几种人体组织的多萜醇显示出向较短链长的转变，以含有18和19个异戊二烯单元的同系物为主。